Table 2 Bacterial strains and plasmids used.
Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
Strains | Â | Â |
   O. tritici SCII24T | As(III)r, As(V)r, Sb(III)r | LMG |
   O. tritici 5bvl1 | As(III)s, As(V)r, Sb(III)s | This laboratory |
   O. grignonense OgA9aT | As(III)s, As(V)r, Sb(III)s | LMG |
   O. anthropi LMG 3331T | As(III)s, As(V)r, Sb(III)s | LMG |
   O. intermedium LMG 3301T | As(III)s, As(V)r, Sb(III)s | LMG |
   E. coli XL1-Blue | rec A1 end A1 gyr A96 thi hsd R17 (r-km+k) sup E44 rel A1 lac [F' pro AB+ lac IqZΔM15::Tn 10(tetr)] | Stratagene |
   E. coli AW3110 | K-12 F- IN(rrnD-rrnE)Δars::cam | 3 |
Plasmids | Â | Â |
   pGEM-T Easy | Ampr lacZ, cloning vector | Promega |
   pUC18 | Ampr lacZ, cloning vector | Invitrogen |
   pTRC 99A | Ampr, ptrc promotor, expression vector | Amersham |
   parsR1DAcbsB | Fragment of genes arsR1, arsD, arsA, arsB and cbs domain cloned into pTRC 99A | This study |
   parsDAcbsB | Fragment of genes arsD, arsA and arsB and cbs domain cloned into pTRC 99A | This study |
   parsAcbsB | Fragment of genes arsA, arsB and cbs domain cloned into pTRC 99A | This study |
   parsAcbs | Fragment of genes arsA and cbs domain cloned into pTRC 99A | This study |
   parscbsB | Fragment of genes arsB and cbs domain cloned into pTRC 99A | This study |
   parsA | Fragment of gene arsA cloned into pTRC 99A | This study |
   parsB | Fragment of gene arsB cloned into pTRC 99A | This study |
   parsR2C1Acr3C2HR3 | Fragment of genes arsR2, arsC1, Acr3, arsC2, arsH and arsR3 cloned into pTRC 99A | This study |
   parsR2C1Acr3C2H | Fragment of genes arsR2, arsC1, Acr3, arsC2 and arsH cloned into pTRC 99A | This study |
   parsR2C1Acr3C2 | Fragment of genes arsR2, arsC1, Acr3 and arsC2 cloned into pTRC 99A | This study |
   parsR2C1Acr3 | Fragment of genes arsR2, arsC1 and Acr3 cloned into pTRC 99A | This study |
   parsAcr3C2HR3 | Fragment of genes Acr3, arsC2, arsH and ars R2 cloned into pTRC 99A | This study |
   parsAcr3C2H | Fragment of genes Acr3, arsC2 and ars H cloned into pTRC 99A | This study |
   parsAcr3C2 | Fragment of genes Acr3 and arsC2 cloned into pTRC 99A | This study |
   parsC1 | Fragment of gene arsC1 cloned into pTRC 99A | This study |
   parsC2 | Fragment of gene arsC2 cloned into pTRC 99A | This study |