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Table 2 Sensitivity of the LAMP assay for CT-producing V. cholerae

From: Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification

Strain

Specimens

 

Dilutions of cultures for the assays

   

10-2

10-3

10-4

10-5

CT-producing V. cholerae

      

13H173

Pure cultures

CFU per reaction

288.8

28.8

2.9

0.3

  

LAMP

+

+

+

± (2/3)

  

PCR

+

+

± (1/3)

-

 

Spiked human feces

CFU per reaction

144.4

14.4

1.4

0.1

  

LAMP

+

+

+

-

  

PCR

+

+

-

-

  1. +, triplicate assay showed all positive.
  2. ±, triplicate assay showed both positive and negative (positive number/tested number).
  3. -, triplicate assay showed all negative.
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BMC Microbiology

ISSN: 1471-2180

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