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Table 1 Results of the LAMP assay

From: Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification

Species

Strains

No. of strains

LAMP results

Production of CT/LT

Source

Vibrio cholerae O1

 

26

   
 

61H151

 

+

+

Human feces, Japan?, 1986

 

4H77

 

+

+

Human feces, Thailand, 1992

 

5H176

 

+

+

Human feces, Indonesia, 1993

 

5H332

 

+

+

Human feces, Indonesia, 1993

 

6H58

 

+

+

Human feces, Thailand, 1994

 

6H62

 

+

+

Human feces, Unknown, 1994

 

6H346

 

+

+

Human feces, Indonesia, 1994

 

7H164

 

+

+

Human feces, Indonesia, 1995

 

7H285

 

+

+

Human feces, Japan, 1995

 

8H215

 

+

+

Human feces, Japan, 1996

 

9H3

 

+

+

Human feces, Thailand, 1997

 

10H1

 

+

+

Human feces, China, 1998

 

10H169

 

+

+

Human feces, Philippines, 1998

 

10H664

 

+

+

Human feces, Philippines, 1999

 

11H215

 

+

+

Human feces, India, 1999

 

11H558

 

+

+

Human feces, Madagascar, 2000

 

13H59

 

+

+

Human feces, Indonesia, 2001

 

13H82

 

+

+

Human feces, Japan, 2001

 

13H173

 

+

+

Human feces, Japan, 2001

 

13H257

 

+

+

Human feces, Indonesia, 2001

 

15H245

 

+

+

Human feces, Thailand, 2004

 

17H16

 

+

+

Human feces, Indonesia, 2005

 

18H24

 

+

+

Human feces, India, 2006

 

62H92

 

-

-

Human feces, Japan?, 1987

 

2H283

 

-

-

Human feces, Indonesia, 1991

 

11H259

 

-

-

Human feces, Egypt/Greece, 1999

Vibrio cholerae O139

 

13

   
 

236-93

 

+

+

Human, India, 1993

 

1034-93

 

+

+

Human, Pakistan, 1993

 

183-93

 

+

+

Human, Bangladesh, 1993

 

21-93

 

+

+

Human, Chennai, India, 1993

 

65-93

 

+

+

Human, Kolkata, India, 1993

 

481-93

 

+

+

Human, Thailand, 1993

 

495-96

 

+

+

Human, Nepal, 1996

 

99-93

 

+

+

Human, Vellore, India, 1993

 

147-93

 

+

+

Human, Madurai, India, 1993

 

VC-23

 

+

+

Unknown, 1995

 

333-93

 

+

+

Pond, India, 1993

 

1033-93

 

-

-

Human, Sri Lanka, 1993

 

Arg-3

 

-

-

Human, Argentine

Vibrio cholerae non-O1/non-O139

 

8

   
 

61H37

 

-

-

Human feces, Thailand, 1986

 

3H264

 

-

-

Human feces, Indonesia, 1992

 

5H231

 

-

-

Human feces, Indonesia, 1993

 

9H237

 

-

-

Human feces, China, 1997

 

9H300

 

-

-

Human feces, India, 1997

 

12H207

 

-

-

Human feces, 2000

 

19H149

 

-

-

Human feces, 2007

 

3H222

 

-

-

Clam, Japan, 1991

LT-producing E. coli

 

7

- (0/7)

+ (7/7)

Human feces, Japan

V. parahaemolyticus

 

6

-

ND

Human feces, Japan

V. vulnificus

 

3

-

ND

IFO15645T and human feces, Japan

V. alginolyticus

 

2

-

ND

IFO15630T and unknown source

V. fluvialis

 

1

-

ND

Human feces

V. furnissii

 

1

-

ND

Human feces

V. harveyi

 

1

-

ND

IFO15634T

V. metschnikovii

 

1

-

ND

Human feces

V. mimicus

 

1

-

ND

Human feces

Other bacteria

 

30

-

ND

Described in Methods.

  1. +, positive reaction; -, negative reaction (positive number/strain number tested); ND, not determined.