The interplay of StyR and IHF regulates substrate-dependent induction and carbon catabolite repression of styrene catabolism genes in Pseudomonas fluorescens ST

Table 2 Oligonucleotides used in this study.

Name	Sequence (5'-3')^a	Position^b	Site^c
P1/FW	GCTCTAGA GGTGTAGTAAATATAAGT	-115	Xba I
P169/FW	GCTCTAGA GGTGTAGTAAATATTAGT	-115	Xba I
P87/FW	TAAATATAAGTAA ATGATTTTTAAT	-108	-
P89/FW	TAAATATT AGTTTATGAA TTTTAAT	-108	-
P86/RV	ATTAAAAATCATTT ACTTATATTTA	-84	-
P88/RV	ATTAAAAT TCATAAACTA ATATTTA	-84	-
P17/RV	GGGGTACC TACGTAGTAGTAGTGG	+274	Kpn I
P35/FW	GGGAATTC CGTTGACTGCTTCGGG	-145	Eco RI
P56/RV	AAACTGCAG AGCTAACACCAGCAGC	-8	Pst I

^a introduced restriction sites are underlined; the nucleotides in bold-face and underlined correspond to the A → T and T → A introduced substitutions with respect to the styA wild-type sequence.
^b distance in bp from PstyA transcription start point [10].
^c restriction recognition sites.

ISSN: 1471-2180