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Table 2 Oligonucleotides used in this study.

From: The interplay of StyR and IHF regulates substrate-dependent induction and carbon catabolite repression of styrene catabolism genes in Pseudomonas fluorescens ST

Name Sequence (5'-3')a Positionb Sitec
P1/FW GCTCTAGA GGTGTAGTAAATATAAGT -115 Xba I
P169/FW GCTCTAGA GGTGTAGTAAATATTAGT -115 Xba I
P87/FW TAAATATAAGTAA ATGATTTTTAAT -108 -
P89/FW TAAATATT AGTTTATGAA TTTTAAT -108 -
P86/RV ATTAAAAATCATTT ACTTATATTTA -84 -
P88/RV ATTAAAAT TCATAAACTA ATATTTA -84 -
P17/RV GGGGTACC TACGTAGTAGTAGTGG +274 Kpn I
P35/FW GGGAATTC CGTTGACTGCTTCGGG -145 Eco RI
P56/RV AAACTGCAG AGCTAACACCAGCAGC -8 Pst I
  1. a introduced restriction sites are underlined; the nucleotides in bold-face and underlined correspond to the A → T and T → A introduced substitutions with respect to the styA wild-type sequence.
  2. b distance in bp from PstyA transcription start point [10].
  3. c restriction recognition sites.