Skip to main content

Table 1 Bacterial strains and plasmids used in this study.

From: The interplay of StyR and IHF regulates substrate-dependent induction and carbon catabolite repression of styrene catabolism genes in Pseudomonas fluorescens ST

Strains and Plasmids Relevant characteristics and plasmids contruction or source Reference
Strains   
P. fluorescens ST Sty+ [39]
E. coli DH5α endA1 hsdR17 supE44 thi-1 recA1 gyrA96 (NalR) relA1 Δ (lacIZYA-argF)U169 deoR(Φ80dlacZ ΔM15) Bethesda Res. Lab.
Plasmids   
pBluescript II KS+ Cloning vector; ApR; 2.9 Kb. Stratagene
pPR9TT lacZ promoter probe vector; ApR; CmR. [40]
pTE50 pTZ19R derivative containing a P. fluorescens ST chromosomal fragment carrying the genes coding for styR, styA and styB; ApR. [7]
pRK2013 Helper plasmid; ColE1 replicon; Mob+; Tra+; KmR. [37]
pSTY1/2 155 bp fragment, encompassing nucleotides -145 to -8, with respect to the styA transcription start point ligated to the Eco RI-Pst I sites of pBluescript II KS+; ApR. [16]
pPR9Pa1 355 bp fragment, encompassing nucleotides -115 to +240, with respect to the styA transcription start point, ligated to the Xho I-Bam HI sites of pPR9TT; ApR; CmR. [20]
pPR9STY1mut Same construct as pPR9Pa1, but the cloned sequence carries a double mutation in positions -97 and -96 from the styA trascription start point; ApR; CmR. This study
pPR9IHFmut Same construct as pPR9Pa1, but the cloned sequence carries a double mutation in positions -101 and -91 from the styA trascription start point; ApR; CmR. This study