Strains and Plasmids | Relevant characteristics and plasmids contruction or source | Reference |
---|---|---|
Strains | Â | Â |
P. fluorescens ST | Sty+ | [39] |
E. coli DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA96 (NalR) relA1 Δ (lacIZYA-argF)U169 deoR(Φ80dlacZ ΔM15) | Bethesda Res. Lab. |
Plasmids | Â | Â |
pBluescript II KS+ | Cloning vector; ApR; 2.9 Kb. | Stratagene |
pPR9TT | lacZ promoter probe vector; ApR; CmR. | [40] |
pTE50 | pTZ19R derivative containing a P. fluorescens ST chromosomal fragment carrying the genes coding for styR, styA and styB; ApR. | [7] |
pRK2013 | Helper plasmid; ColE1 replicon; Mob+; Tra+; KmR. | [37] |
pSTY1/2 | 155 bp fragment, encompassing nucleotides -145 to -8, with respect to the styA transcription start point ligated to the Eco RI-Pst I sites of pBluescript II KS+; ApR. | [16] |
pPR9Pa1 | 355 bp fragment, encompassing nucleotides -115 to +240, with respect to the styA transcription start point, ligated to the Xho I-Bam HI sites of pPR9TT; ApR; CmR. | [20] |
pPR9STY1mut | Same construct as pPR9Pa1, but the cloned sequence carries a double mutation in positions -97 and -96 from the styA trascription start point; ApR; CmR. | This study |
pPR9IHFmut | Same construct as pPR9Pa1, but the cloned sequence carries a double mutation in positions -101 and -91 from the styA trascription start point; ApR; CmR. | This study |