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Figure 3 | BMC Microbiology

Figure 3

From: Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner

Figure 3

The effect of preabsorption with GST fusion proteins on human antibody reactivity with the microplate-immobilized fusion proteins. The pooled human serum was 5-fold serially diluted as indicated along the X-axis and reacted with the microplate-immobilized GST-pgp3 (filled square), GST-CPAF (open square), GST-MOMP (open triangle) and GST-HSP60 (filled triangle). The reactivity was expressed as OD at 405 nm as indicated along the Y-axis. The pre-absorption of the pooled antiserum with GST-pgp3 fusion protein only blocked the human antibody binding to the pgp3 but not other fusion proteins (panel b) while the pre-absorption with GST-CPAF fusion protein only blocked antibody binding to CPAF but not other fusion proteins (panel c).

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BMC Microbiology

ISSN: 1471-2180

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