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Figure 1 | BMC Microbiology

Figure 1

From: Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner

Figure 1

Reactivity of human antibodies with chlamydial fusion proteins. The GST fusion proteins were precipitated with glutathione-agarose bead from bacterial lysates and the precipitates were checked in SDS polyacrylamide gel for quality (panel a). The same lysates were used as the source of fusion proteins in ELISA. 15 patient serum antibodies (along the Y-axis of panel b) were each diluted at 1:500 and reacted with the bacterial lysates containing GST-chlamydial fusion proteins or GST alone (listed both on the top and in the bottom of the figure). Each positive reaction was marked with a horizontal bar (panel b) and the number of sera reacted with each fusion protein was plotted in panel c. The average ODs were calculated (panel d). The 15 sera were pooled and measured against the fusion proteins (panel e). A pooled serum from 8 health individuals (pooled negative serum) was used to similarly react with the fusion proteins (panel f). Note that pgp3 and CPAF fusion proteins were recognized by most antiesra and with the highest titers.

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