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Figure 1 | BMC Microbiology

Figure 1

From: Formate hydrogenlyase in the hyperthermophilic archaeon, Thermococcus litoralis

Figure 1

The genomic organization and transcription of the fdh-mhy operon. A. Restriction map of the isolated 10.5 kb long T. litoralis genomic fragment and the genetic organization of the fdh-mhy genes. The main subclones are indicated by arrows. B. The products of the RT-PCR experiments performed to prove that the fdh-mhy genes are located on a single mRNA. cDNA was made using the fhl8frC1R primer, and the amplified regions is indicated with bold lines. Lane 1,17: GeneRuler™ 1 kb DNA Ladder (Fermentas). Templates: lane 2,5,8,11,14: genomic DNA template, lane 3,6,9,12,15: RT(-) experiment lacking reverse transcriptase, lane 4,7,10,13,16: RT(+) experiment. Primers: lane 2–4: fhlBR2-fhlBN1, lane 5–7: fhl02N-fhl03R, lane 8–10: fhl802N-fhl07R, lane 11–13: fhl05R-fhl09N, lane 14–16: fhl8frC1R-fhl12N.

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