Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Sidhu, Vishaldeep K; Vorhölter, Frank-Jörg; Niehaus, Karsten; Watt, Steven A

doi:10.1186/1471-2180-8-87

Table 1 Proteins identified in the OMV fraction of cultures grown in M9 medium.

From: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Section	Protein	Acc. No.	MW(cal)	Size range	Coverage (ESI-MS/MS)	Score (ESI-MS/MS)	Coverage (MALDI)	Score (MALDI)
1	TonB-dependent receptor with signalP	AAM 42139	100858	80–150	10	162	31	134
	Outer membrane protein With signalP	AAM40663	90600		6	113	27	96
	Oar putative outer membrane receptor protein, tonB-dependent with signalP	AAM41771	117723		4	91	11	86
	TonB-dependent outer membrane receptor	AAM41663	91909		6	49	9	89
	FpvA TonB-dependent outer membrane ferripyoverdine receptor with signalP	AAM42628	81083		6	59	8	60
	HrcV (HrcV)	AAM40527	69015		9	32	12	54
	HrpF (HrpF protein)	AAM40515	98836		11	30	19	92
	AcrD Outer membrane efflux protein	AAM41427	130193		8	27	11	102
2	FadL outer membrane fatty acid porin with signalP	AAM39336	47455	60–80	13	235	-	-
	Egl exported cellulase with signalP	AAM42791	52209		7	140	18	88
	Avirulence protein with signalP	AAM43445	67281		5	27	9	59
	HrcN HrcN protein	AAM40534	47759		5	30	7	52
	ffh Signal recognition particle protein	AAM40492	49312		7	27	-	-
	AvrBs2 (avirulence protein)	AAM39371	78471		3	27	-	-
3	OmpA family outer membrane protein with signalP	AAM40245	39338	40–60	27	405	21	143
	AvrBs1 avirulence protein AvrBs1	AAM41388	49789		6	29	8	61
	XynB exported xylan 1,4-beta-xylosidase with signalP	AAM43196	58311		3	32	-	-
	HrcU HrcU protein	AAM40528	38694		6	27	6	57
4	OmpA family outer membrane protein with signalP	AAM40245	39338	30–40	32	402	19	96
	VirB6 protein	AAM42571	38386		6	32	5	56
	Hrc U HrcU protein	AAM40528	38694		8	30	8	58
	RlpA Exported rare lipoprotein A With signalP	AAM42727	46235		6	35	-	-
	HrpW* (HrpW protein)	AAM40517	33372		2	28	11	59
5	Putative exported protein	AAM40085	35080	25–30	8	53	9	68
	OmpW3 OmpW family outer membrane protein	AAM42791	23494		7	41	10	63
	*RlpB Rare lipoprotein B with signalP	AAM40532	22959		10	31	-	-
	Lipoprotein precursor with signalP	AAM41856	26209		4	26	-	-
6	OmpW3 OmpW family outer membrane protein	AAM42791	23494	20–25	8	58	10	72
	HrpB4 HrpB4 protein	AAM40532	22919		4	29	4	52
	HpaH Putative transglycosylase HpaH	AAM40539	21109		3	29	6	58
7	Putative exported protein with signalP	AAM40594	10647	15–20	15	171	22	127
	XpsH General secretion pathway protein	AAM39979	18208		20	47	17	61
	UptD outer membrane protein with signalP	AAM39910	17116		15	39	18	83
	HrpE HrpE protein	AAM40519	9749		30	30	32	56

The section indicates the gel-section excised from the corresponding SDS-PAGE. The values indicated under MW(cal) gives the calculated molecular weight of the identified proteins. The size range relates the excised gel-section with regard to the molecular weight standard on the SDS-PAGE. Score (MOWSE-score) and coverage (sequence coverage) relate to the two approaches (ESI-MS/MS or MALDI-TOF-MS) utilized for the identification of the proteins on the SDS-PAGE.

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