Figure 1

Electron micrgraphes of Xcc cell and OMV preparations. (A) Electron micrograph of 1% (w/v) uranyl acetate stained outer membrane vesicles extracted from the cell free supernatant of Xanthomonas campestris pv campestris strain B100 at a 30 k fold magnification (OMVs highlighted by arrows). (B) Immunogold electron microscopy of 1% (w/v) uranyl acetate stained Xcc cell at 14 k fold magnification after incubation with an anti-Xcc B100 antibody which was detected by a 10 nm gold particle bound to a goat anti-rabbit antibody (gold-labelled OMV highlighted by arrows). (C) Immunogold electron micrograph of 1% (w/v) uranyl acetate stained OMVs at 27 k fold magnification after incubation with an anti-Xcc B100 antibody detected with a 10 nm gold particle bound to an goat anti-rabbit antibody.