Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis

Table 2 Optimised primer and probe concentrations determined for the seven species defining MGB assays.

MGB assay	Final Forward primer concentration (nM)	Final Reverse primer concentration (nM)	Final VIC labelled probe concentration (nM)	Final FAM labelled probe concentration (nM)
B. abortus	500	700	300	100
B. melitensis	700	1100	200	100
B. ovis	300	300	250	50
B. suis bv1–4	300	300	200	100
B. canis	700	1100	250	200
Marine mammal Brucella	500	1100	300	50
B. neotomae	500	700	200	100

Primer and probe concentrations were optimised to give the greatest amount of discrimination with the least background using the minimum amounts of the various components.

ISSN: 1471-2180