Figure 6
Discrimination of the Brucella genus defining assays. Application of the two 16S rRNA based probe pairs in distinguishing isolates from the genus Brucellae from other α-proteobacteria. a. Results generated by the 16SrRNA771/778 probe pair when used against 5 species of Ochrobactrum (including three strains of O. anthropi) and representatives of all the known Brucella species and biovars. This reaction separates Brucella isolates from all other except O. anthropi ATCC49237. b. Results generated with the application of 16SrRNA1055 probe pair illustrating the use of this assay to further separate O. anthropi ATCC49237 from Brucella isolates.