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Figure 1 | BMC Microbiology

Figure 1

From: Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis

Figure 1

Seven Species defining MGB assay profile. Example of the multiple outcome assay used to identify twelve Brucella isolates. Each species-defining assay is run in rows (A-G) with samples run in columns. The green PCR profiles represent reactions with the VIC labelled probe, representing the species-specific probe in each probe pair. The blue PCR profiles represent reactions with the FAM labelled probes, representing the reaction with the alternate state (non species-specific) allele probe in each probe pair. The identity of each of the isolates 1–12 is indicated by a red dot where an isolate generates a positive PCR reaction with a VIC-labelled probe.

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