Figure 1From: Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysisSeven Species defining MGB assay profile. Example of the multiple outcome assay used to identify twelve Brucella isolates. Each species-defining assay is run in rows (A-G) with samples run in columns. The green PCR profiles represent reactions with the VIC labelled probe, representing the species-specific probe in each probe pair. The blue PCR profiles represent reactions with the FAM labelled probes, representing the reaction with the alternate state (non species-specific) allele probe in each probe pair. The identity of each of the isolates 1–12 is indicated by a red dot where an isolate generates a positive PCR reaction with a VIC-labelled probe.Back to article page