|
Locus
|
Primer
|
Dye-Sequence
|
DNA sequence length in bpa (size ABI 310b)
|
|---|
|
SENTR1
|
SENTR1-F
|
HEX-GCAACAGCAGCAGCAACAG
|
440 (415)
|
| |
SENTR1-R
|
CCGAGCTGAGATCGCCAAG
| |
|
SENTR2
|
SENTR2-F
|
FAM-CACTGGACGATCTGGATTTCTC
|
519 (510)
|
| |
SENTR2-R
|
GTCGCCGTTACGCATCAAC
| |
|
SENTR3
|
SENTR3-F
|
TET-CTAAACAAGCCGCTCATCCG
|
494 (480)
|
| |
SENTR3-R
|
ACAACCTGCTGCTGTGCTG
| |
|
SENTR4
|
SENTR4-F
|
HEX-GACCAACACTCTATGAACCAATG
|
120 (120)
|
| |
SENTR4-R
|
ACCAGGCAACTATTCGCTATC
| |
|
SENTR5
|
SENTR5-F
|
FAM-CACCGCACAATCAGTGGAAC
|
271 (270)
|
| |
SENTR5-R
|
GCGTTGAATATCGGCAGCATG
| |
|
SENTR6
|
SENTR6-F
|
TET-ATGGACGGAGGCGATAGAC
|
180 (177)
|
| |
SENTR6-R
|
AGCTTCACAATTTGCGTATTCG
| |
|
SENTR7
|
SENTR7-F
|
FAM-ACGATCACCACGGTCACTTC
|
135 (133)
|
| |
SENTR7-R
|
CGGATAACAACAGGACGCTTC
| |
|
SE-3
|
SE-3F
|
HEX- CAACAAAACAACAGCAGCAT
|
308 (307)
|
| |
SE-3R
|
GGGAAACGGTAATCAGAAAGT
| |
|
SE-7
|
SE-7F
|
HEX- GATAATGCTGCCGTTGGTAA
|
717 (691)
|
| |
SE-7R
|
ACTGCGTTTGGTTTCTTTTCT
| |
- a based on S. Enteritidis NCTC 13349 [25].
- b size analysed using the capillary sequence detection system ABI 310 (Applied Biosystems)
