Characterization of BbKH500 and BbKH501. (A) Whole-cell lysates of Bb297, BbKH500, and BbKH501 were resolved by SDS-PAGE (12.5% acrylamide) and stained with Coomassie brilliant blue or (B) or transferred to nitrocellulose and assessed by immunoblot analysis with antibodies as noted on the right. (C) PCR analysis of Bb297, BbKH500 and BbKH501 for presence of dbpA. (D) Immunoblot analysis of whole-cell lysates of proteinase K-digested (+) or undigested (-) Bb297 or BbKH501. Antibodies used noted on the right. Molecular mass of markers (M) in panel A are shown in kDa. The arrow at the right in panel A denotes the protein band corresponding to DbpA. DNA size standards (M) in panel C are shown in base pairs.