Figure 4
From: Functional comparison of plasma-membrane Na+/H+ antiporters from two pathogenic Candida species
Immunodetection of GFP-tagged antiporters in S. cerevisiae cells. Cells expressing Sc Nha1-GFP, Ca Cnh1-GFP, Cd Cnh1-GFP and Cp Cnh1-GFP were grown to exponential phase and proteins extracted. After separation of proteins via SDS-PAGE they were transferred to nitrocellulose membrane and detected with anti-GFP antibody.