Figure 1
RFLP analysis of mcrA gene amplicons. 4% (w/v) agarose gel showing representatives of the four unique mcrA gene RFLP types identified in this study. Lane 1: RFLP profile of mcrA gene from Mbb. smithii PST (RFLP profile type A), Lane 2: RFLP profile identified in RFLP analysis of IBS clone library, sequence most closely related to Mbb. oralis and represented in Figure 2 by DC IBS-4, (RFLP profile Type B), Lane 3: RFLP profile generated from Ulcerative Colitis clone library analysis, most closely related to Mbb. smithii and represented in Figure 2 by clone DC UC-14, (RFLP profile Type C), Lane 4: RFLP profile Type D generated from Ulcerative Colitis clone library analysis and represented in Figure 2 by DC UC-6, uncultured methanogen clone, M: Low Weight Molecular DNA Ladder (Promega).