Bacterial loads in isolation positive samples, box plot analysis of Ct values. A, bacterial loads and C. burnetii isolation in real-time PCR positive samples (n = 27). Bacterial loads are shown on the y-axis. "+" in "cell isolation" means isolation success as confirmed by detection of inclusion bodies upon microscopy. B, box plot analysis of threshold cycle values in real-time PCR positive/conventional negative (n = 32) and real-time PCR positive/conventional PCR positive (n = 38) samples. Difference in threshold cycle values are significant (p < 0.05).