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Table 2 DDT sequencing of regions selected based on pilot SS14/Nichols comparison using microarray hybridization experiments

From: Complete genome sequence of Treponema pallidum ssp. pallidumstrain SS14 determined with oligonucleotide arrays

Region no.

ORFa

Size of sequenced region (nt)

Left coordinatea

Right coordinatea

Newly found changes

Confirmation of SNPs identified by CGSb

1

TP0017

848

18454

19301

-

-

2

TP0070

339

75493

75831

-

-

3

TP0094

1011

102879

103889

-

-

4

TP0123

1083

143207

144289

-

-

5

TP0192

748

206663

207410

-

-

6

TP0200

264

210183

210446

-

-

7

TP0291

834

304706

305539

-

-

8

TP0319

1014

334847

335860

-

1 SNP

9

TP0321–TP0322

2640

336149

338788

-

-

10

TP0323

851

338885

339735

-

1 SNP

11

TP0376

806

400903

401708

-

-

12

TP0377

78

401851

401928

-

-

13

TP0516

1533

556351

557883

-

-

14

TP0519

1277

559215

560491

-

-

15

TP0580

1242

630328

631569

-

-

16

TP0587

183

639620

639802

-

-

17

TP0633

776

691437

692212

-

-

18

TP0683

1047

746899

747945

-

-

19

TP0799–TP0800

2168

866136

868303

-

-

20

TP0806

1397

875808

877204

-

-

21

TP0807

165

877407

877571

-

-

22

TP0808

187

877632

877818

-

-

23

TP0877

998

953710

954707

-

2 SNPs

24

TP0933

2023

1013098

1015120

-

-

25

TP0952

1438

1032341

1033778

-

1 SNP

26

TP0961

1216

1041973

1043188

-

-

27

TP0980

975

1063047

1064021

-

-

  1. ORF – open reading frame; nt – nucleotide; SNP – single nucleotide polymorphism; aas described in [3]; bSNPs identified using CGS in these regions were verified by DDT sequencing.
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BMC Microbiology

ISSN: 1471-2180

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