Table 2 Primers
From: Sequence diversity in the A domain of Staphylococcus aureusfibronectin-binding protein A
Primer | Sequence (5' – 3')* |
|---|---|
Flanking primers | Â |
pfnbA Adom F | CCGAAGCTTGTGAAAAACAATCTTAGGTAC |
pfnbA Adom R | CCGGGATCCTATCAATAGCTGATGAATCCG |
Type-specific probe primers | Â |
pfnbA N3 I F | AAAGGTAGTAATCAAATGG |
pfnbA N3 I R | GTTAAGGTATATCCTCTATC |
pfnbA N3 II F | GATAGTGTTACTGTAACGG |
pfnbA N3 II R | TAGCGATTTTCAGGGTATC |
pfnbA N3 III F | TAGTAATTTAGCTGGTGGAC |
pfnbA N3 III R | GATATCCCCCATAAACATAG |
pfnbA N3 IV F | CAAAGCAAATGGAAATGCTC |
pfnbA N3 IVR | AGTTGGTATCCCAAATGAG |
pfnbA N3 V F | GAAAGGTAGCAACTCTAATG |
pfnbA N3 V R | TGTTAATGTATAGCCGTGG |
pQE30 expression vector primers | Â |
pfnbApQE II F | CGCGGATCCGGTACAGATGTGACAAGTAAAC |
pfnbApQE II R | CCGAAGCTTATATTTTCCATCACCATTAGC |
pfnbApQE III F | CGCGGATCCGGTACAGATGTGACAAGTAAAG |
pfnbApQE III R | CCGAAGCTTATTTTTACCATTGCCGTCAG |
pfnbApQE V F | CCGGGATCCGGCACAGATGTTACAAGTAAAG |
pfnbApQE V R | CCGAAGCTTATATTTTCCATCACCATTAGC |
pfnbApQE VI F | CTAGGATCCGGCACAGATGTGACAAGTAAAG |
pfnbApQE VI R | CCGAAGCTTATTTTTACCATTACCATCAGC |
pfnbApQE VII F | CCGGGATCCGGTACAGATGTGACAAGTAAAG |
pfnbApQE VII R | CCGAAGCTTATTTTGACCATTACCATCTGC |