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Figure 6 | BMC Microbiology

Figure 6

From: Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection

Figure 6

(A) Lsa21 binds to ECM components in a dose-dependent manner. Binding of Lsa21 to laminin, collagen IV and plasma fibronectin, as a function of protein concentration (0 to 2,000 nM). Each point represents the mean absorbance value at 492 nm ± the standard deviation of three independent experiments. BSA was included as a negative control. (B) Sugar moiety contribution to the laminin-Lsa21 interaction. Immobilized laminin was treated with sodium metaperiodate (5 to 100 mM) for 15 min at 4°C in the dark. Each point represents the mean absorbance value at 492 nm ± the standard deviation of three independent experiments. Reduction in attachment compared to the level of attachment to untreated laminin was statistically significant: in each case (*), the P value, as measured by the Student two-tailed t -test, was ≤ 0.004.

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