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Figure 5 | BMC Microbiology

Figure 5

From: Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection

Figure 5

Binding of Lsa21 recombinant protein to ECM components. Wells were coated with 1 μg of laminin, collagen Type I, collagen Type IV, cellular fibronectin, plasma fibronectin, and the control proteins BSA and fetuin. Recombinant Lsa21 and Loa22 proteins attachment to those ECM macromolecules was assessed by an ELISA-based assay. One microgram of recombinant protein was added per well. Optical densities were taken at 492 nm. Data represent the mean ± standard error of three independent experiments. For statistical analysis, the attachment of Lsa21 to ECM macromolecules was compared to its binding to BSA by the Student's two tailed t- test (*, P < 0.01).

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