Figure 4

Indirect immunofluorescence staining of intact fixed leptospires. L. interrogans serovar Pomona strain LPF and L. biflexa serovar Patoc strain Patoc 1 were incubated with mouse anti-Lsa21 serum, followed by incubation with a fluorescein isothiocyanate-conjugated goat anti-mouse antibody. Magnification × 1,260.