Table 1 Bacterial strains and plasmids used in this study.

P. fluorescens

SBW25

Wild-type

[17]

PBR840

SBW25ΔpvdL or ΔPflu4387

This study

15F3

PBR840 with mini-Tn phoA3 disruption of secondary siderophore biosynthesis genes, Gm^R

S. Matthijs, unpublished

TR107.1.1^a

SBW25 carrying the pvdS-'lacZ fusion, Sm^R

This study

TR107.2.1^a

SBW25 carrying the pvdL-'lacZ fusion, Sm^R

This study

TR107.4.1^a

SBW25 carrying the fpvI::'lacZ fusion, Sm^R

This study

TR107.5.1^a

SBW25 carrying the fpvR::'lacZ fusion, Sm^R

This study

TR135.1.1^a

SBW25 carrying the fpvA::'lacZ fusion, Sm^R

This study

TR137.2.1

TR107.2.1 with pTr130.1, Gm^R

This study

TR137.3.1

TR107.2.1 with pTr130.2, Gm^R

This study

TR137.4.1

TR107.2.1 with pTr130.3, Gm^R

This study

TR156.1.1

TR107.2.1 with pBroadgate-D, Gm^R

This study

TR138.2.1

TR135.1.1 with pTr130.1, Gm^R

This study

TR138.3.1

TR135.1.1 with pTr130.2, Gm^R

This study

TR138.4.1

TR135.1.1 with pTr130.3, Gm^R

This study

TR156.3.1

TR135.1.1 with pBroadgate-D

This study

TR168.1.1

TR107.5.1 with pTr130.1, Gm^R

This study

TR168.2.1

TR107.5.1 with pTr130.2, Gm^R

This study

TR168.3.1

TR107.5.1 with pTr130.3, Gm^R

This study

TR168.4.1

TR107.5.1 with pBroadgate-D

This study

Plasmids

pUIC3

Integration vector with 'lacZ, Tc^R

[20]

pHP45Ω

Source of the omega cassette, Sm^R

[51]

pIVET-Sm

pUIC3 with tet gene replaced by Sm^r/Sp^r Ω fragment from pHP45Ω, Sm^R

This study

pUIC3–40

pUIC3 containing pvdL deletion fragment

This study

pCR2.1

PCR product cloning vector, Km^R

Invitrogen

pRK2013

helper plasmid, Km^R, Tra⁺

[52]

pTR100.1.1

pIVET-Sm carrying the pvdS-'lacZ fusion

This study

pTR100.2.1

pIVET-Sm carrying the pvdL-'lacZ fusion

This study

pTR100.4.1

pIVET-Sm carrying the fpvI-'lacZ fusion

This study

pTR100.5.1

pIVET-Sm carrying the fpvR-'lacZ fusion

This study

pTR128.1.1

pIVET-Sm carrying the fpvA-'lacZ fusion

This study

pBroadgate

Gateway expression vector with P_lac promoter, Gm^R

Thwaites and Mansfield

pBroadgate-D

pBroadgate with ccdB gene replaced with the linker fragment, Gm^R;

[53]

pTR130.1

pBroadgate::pvdS, Gm^R

This study

pTR130.2

pBroadgate::fpvI, Gm^R

This study

pTR130.3

pBroadgate::fpvR, Gm^R

This study