Table 1 Oligonucleotides used in the RT-PCR, PCR, Northern blot, and 5'RACE experiments
From: Transcription of the extended hyp-operon in Nostocsp. strain PCC 7120
Gene | RT-primer sequence 5'-3' | Forward primer sequence 5'-3' | Reverse primer 5'-3 |
|---|---|---|---|
RT-PCR AND PCR | |||
asr0689 | - | tggttagttgtagccacgctta | tag |
asr0690 | - | cggcgaggttatttttgaag | tag |
alr0691 | tag + ctggggtggtcaatcaagtt | ttggcgaggataaccgatag | tag |
alr0692 | tag + cttgggataacgtcaaagtagaa | aaaagggcgattgaggattt | tag |
alr0693 | tag + tacccttagcattctg | tgggacaaaggaatttttcg | tag |
hypF.1 | tag + ccttgatatggtgtcctgat | cgactgaggaaattcgagtg | tag |
hypF.2 | tag + ttcatcaccacatacc | aagggaattggtggttttca | tag |
hypC | tag + gcggcgatttcttgtaataat | tcaccgacattaaccacaaa | tag |
hypD | - | ccaattgttgtttccggttt | tag |
hypE | tag + tcgtccttttggatgagattt | attttaattgcccgtggtga | tag |
hypA | - | tgaatatgctcaaggctcaaaa | tag |
hypB | tag + tggaagcatccaaatgacaa | gggaacaggttgtcatttgg | tag |
asr0701 | tag + gctgggaaagcttgatatat | acgctattacatcaaattct | tag |
alr0702 | tag + taaaccgctattggggtcag | tggtgaagtgattgggatga | tag |
NORTHERN BLOT | |||
asr0689 | tggttagttgtagccacgctta | cccagacccaaaacaatagc | |
alr0693 | tgggacaaaggaatttttcg | aaaacttttctgccccaaca | |
hypF | aagggaattggtggttttca | tcaaatgatgcaaaggcgta | |
hypA-hypB | tgaatatgctcaaggctcaaaa | tgtgggggtatttgattggt | |
IDENTIFICATION OF TRANSCRIPTION START POINTS, 5'RACE | |||
asr0689 | taagcgtggctacaactaacca | ||
alr0693 | tcaacaaccgacgattacca | ||
hypF | cagcatccacatcatccaac | ||
hypC | agcccaaagcgaagaataca | ||