Figure 1

(A) Physical map of the extended hyp -operon, covering a region of almost 14 kb, in Nostoc sp. strain PCC 7120. Apart from the hyp-genes (depicted in black) the region also includes five genes upstream of hypF (depicted in grey), the two genes downstream of hypB (depicted in grey), and asr0697 (between hypD and hypE, depicted in grey). The overlapping cDNA of the region are marked a-i. Identified transcriptional start points, TSP, of the extended hyp-operon located upstream of asr0689, hypF and hypC are marked with arrows. Number R1–R6 represents the intergenic regions where conserved sequences were found in the extended hyp-operon. (B) Agarose gel showing the overlapping amplified PCR products, 1–22, of the RT-PCR experiments, a-i. (C) Primer pairs (1–22) used in the mapping of the extended hyp-operon consisting of overlapping cDNA (a-i). The size indicates the expected length of the PCR-product of the forward primer together with the reverse tag-primer.