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Table 1 Bacterial strains used in this study

From: The influence of in vitro fitness defects on pneumococcal ability to colonize and to cause invasive disease

Pneumococcal strains

Description

Source or reference

Clinical isolates

  

1-A (BHN31)

Serotype 1 invasive isolate of ST306

[12]

1-B (BHN32)

Serotype 1 invasive isolate of ST228

[12]

6B-A (BHN49)

Serotype 6B invasive isolate of ST138

[12]

6B-B (BHN50)

Serotype 6B carrier isolate of ST176

[12]

7F-A (BHN55)

Serotype 7F invasive isolate of ST191

[12]

7F-B (BHN56)

Serotype 7F carrier isolate of ST191

[12]

14-A (BHN78)

Serotype 14 invasive isolate of ST124

[12]

14-B (BHN83)

Serotype 14 carrier isolate of ST555

[12]

19F-A (BHN100)

Serotype 19F carrier isolate of ST162

[12]

19F-B (BHN97)

Serotype 19F carrier isolate of ST425

[12]

Laboratory strains

  

TIGR4 (BHN126)

Wild-type strain of serotype 4 (EmS SmS)

[16]

TIGR4S (BHN160)

TIGR4 spontaneous streptomycin resistant mutant (SmR)

This study

TIGR4ΔychF (BHN168)

ychF (SP0004)::erm (EmR)

This study

TIGR4ΔrluD (BHN169)

rluD (SP0929)::erm (EmR)

This study

TIGR4Δsmf (BHN170)

smf (SP1266)::erm (EmR)

This study

TIGR4ΔhemK (BHN171)

hemK (SP1021)::erm (EmR)

This study

TIGR4ΔyebC (BHN172)

yebC (SP1922)::erm (EmR)

This study

TIGR4ShemK- (BHN173)

Point mutations in hemK (SP1021) (SmR)

This study

TIGR4SyrdC- (BHN174)

Point mutations in yrdC (SP1022) (SmR)

This study

TIGR4SSP1023- (BHN175)

Point mutations in SP1023 (SmR)

This study