Table 2 Sequence characteristics of the hybridization probes on the AT genotyping microarray
Probe | Sequence* | Binding site* | Length | GC/% | Tm/°C |
|---|---|---|---|---|---|
VD2-01 | GGAATTGCTGGAAATAGCGAAAGTAATGC | AF269269.1 [466:494] | 29 | 41 | 60.9 |
VD2-02 | GGTTTTCAGCTGCAAGCTCAATCTC | M73035.1 [554:578] | 25 | 48 | 60.3 |
VD2-03 | GGTTTTCAGCTACCAACTCAACCTCT | AF269265.1 [488:513] | 26 | 46 | 60.3 |
VD2-04 | GGTTTTCAGCTACCAGCTCAACCT | AY327465.1 [488:511] | 24 | 50 | 60.3 |
VD2-11 | AGGAAACACCTTAACAAATGACCGACT | AJ310735.1 [483:509] | 27 | 41 | 60.2 |
VD2-12 | GCTACCAACTCAACCTCTACCGATCT | AF269265.1 [496:521] | 26 | 50 | 61.0 |
VD2-13 | GAGCCTCTTTATCAGAGCAACTTCCA | AJ243525.1 [324:349] | 26 | 46 | 60.0 |
VD2-14 | AGCTCCTTAACAAATGACCAACTTCCC | AF269260.1 [478:504] | 27 | 44 | 60.7 |
VD2-15 | CCAGCTCAACCTCTACCGAGCT | AY327465.1 [500:521] | 22 | 59 | 61.0 |
VD2-21 | CCGTAGCAGCTGATCAACTTCCA | AF269259.1 [482:504] | 23 | 52 | 60.1 |
VD2-22 | GCAGTTAGTACCGATCTTCCAAAGCA | AF269268.1 [505:530] | 26 | 46 | 60.5 |
VD2-23 | TCAATAATCAACTTCCAAACGTAGCCATCA | AF269266.1 [494:523] | 30 | 37 | 60.6 |
VD2-24 | CTCTACCGAGCTTCCAATGCAACT | AY327465.1 [510:533] | 24 | 50 | 60.2 |
VD2-25 | CTCTACCGATCTTCCAACGCAACT | AF269281.1 [738:761] | 24 | 50 | 60.0 |
VD2-31 | CGATCTTCCAACGCAACTTCCTAAC | AF269281.1 [744:768] | 25 | 48 | 59.7 |
VD2-32 | CGATCTTCCAATGCAACTTCCTAACG | M73035.1 [582:607] | 26 | 46 | 59.9 |
VD2-33 | CGATCTTCCAAAGCAACTTCCTAACG | AF269268.1 [516:541] | 26 | 46 | 59.8 |
VD2-34 | CGAGCTTCCAATGCAACTTCCTAAC | AY327465.1 [516:540] | 25 | 48 | 59.9 |
VD4-01 | TGGCTACTGCTGTTTTAGACGCA | AF269269.1 [923:945] | 23 | 48 | 59.9 |
VD4-02 | TGGCCTCTGCTGTTATGAACTTGAC | AF269260.1 [914:938] | 25 | 48 | 60.5 |
VD4-03 | AGCCGCTGCTGTTTTGAACTTGA | AF269259.1 [915:937] | 23 | 48 | 61.2 |
VD4-11 | CCCAAGCCTTATAGGATCAACCACTG | M73035.1 [1047:1072] | 26 | 50 | 60.3 |
VD4-12 | CCAAGCCTTCTAGGATCAACCACTG | AF269265.1 [982:1006] | 25 | 52 | 60.3 |
VD4-13 | CCAAGCCTTGTAGGATCAACCACTG | M73035.1 [1048:1072] | 25 | 52 | 60.8 |
VD4-21 | CCTTATAGGATCAACCACTGCTTTGCC | M73035.1 [1053:1079] | 27 | 48 | 61.1 |
VD4-22 | CCTTCTAGGATCAACCACTACTTTGCC | AF269262.1 [927:953] | 27 | 48 | 60.4 |
VD4-23 | CCTTTTAGGGGAAGCCACAAATTTAGACT | AJ243525.1 [799:827] | 29 | 41 | 60.7 |
VD4-24 | CCTTCTAGGATCAACCACTGCTTTGC | AF269265.1 [987:1012] | 26 | 50 | 61.2 |
VD4-25 | AGGGCAAGCTACAAATTTAGATACTAGCA | AJ310735.1 [969:997] | 29 | 38 | 59.7 |
VD4-31 | ACTACTTTGCCCAATAATGGTGGTAAGG | AF269262.1 [943:970] | 28 | 43 | 60.4 |
VD4-32 | CTGCTTTGCCCAATAATAATAGTGGTAAGG | Y16561.1 [1004:1033] | 30 | 40 | 59.8 |
VD4-33 | TGCTTTGCCCAATAATAGTGGTAAGGA | M73035.1 [1071:1097] | 27 | 41 | 59.8 |
VD4-34 | AGCTTTAGATGCTAGCAACAAATTCTGC | AF269259.1 [972:999] | 28 | 39 | 60.1 |
VD4-35 | GCTTTGCCCAATAATGCTGGTAAGG | AY327465.1 [1006:1030] | 25 | 48 | 60.2 |
VD4-36 | TGTCGACGGTACCAATACTTACTCTGA | AF269266.1 [981:1007] | 27 | 44 | 60.3 |
mean | 25 | 47 | 60.4 |