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Table 1 Genes involved in B. cereus ATCC14579 glucose metabolism and their putative CRE-sites

From: Assessment of CcpA-mediated catabolite control of gene expression in Bacillus cereusATCC 14579

Gene

Operon¶

Annotation

BC nr.

RZC nr.

ΔccpA/WT †

CRE‡

     

early

mid

 

Glycolysis

ptsG#

1

PTS system, glucose-specific IIABC component

BC4050

RZC06596

0.52

1.12

2

ptsH#

2

Phosphocarrier protein HPr

BC4049

RZC00081

0.56

1.10

+

ptsI#

3

Phosphoenolpyruvate-protein phosphotransferase

BC4048

RZC06595

0.66

1.16

1

pgi

 

Glucose-6-phosphate isomerase

BC4898

RZC03793

0.31

0.20

1

pfkA

1

6-phosphofructokinase

BC4600

RZC02283

0.45

0.47

-

pykA

2

Pyruvate kinase

BC4599

RZC01414

0.29

0.33

-

fbaA

 

Fructose-bisphosphate aldolase

BC5335

RZC03557

0.39

0.52

2

cggR#

1

Central glycolytic genes regulator

BC5141

RZC08032

0.49

0.73

-

gapA#

2

Glyceraldehyde-3-phosphate dehydrogenase

BC5140

RZC00210

0.36

0.59

-

pgk* #

3

Phosphoglycerate kinase

BC5139

RZC00211

0.49

0.41

-

pgk* #

3

Phosphoglycerate kinase

BC5138

RZC08031

0.53

0.46

-

tpiA#

4

Triose-phosphate isomerase

BC5137

RZC08030

0.62

0.51

-

pgmA#

5

Phosphoglycerate mutase

BC5136

RZC05843

0.48

0.38

-

eno#

6

Phosphopyruvate hydratase

BC5135

RZC02971

0.42

0.51

-

gapN

 

NADP-dependent glyceraldehyde-3-P dehydrogenase

BC0868

RZC03841

0.31

0.26

2

Pyruvate dehydrogenase

pdhA#

1

Pyruvate dehydrogenase (acetyl-transferring)

BC3973

RZC01920

1.24

0.77

2

pdhB#

2

Pyruvate dehydrogenase (acetyl-transferring)

BC3972

RZC01921

1.47

0.79

+

pdhC#

3

Dihydrolipoyllysine-residue acetyltransferase

BC3971

RZC06754

1.52

0.95

+

pdhD#

4

Dihydrolipoyl dehydrogenase

BC3970

RZC07919

1.28

1.01

+

Overflow metabolism

alsS§

1

Acetolactate synthase large subunit

BC0883

RZC01898

1.04

0.54

2

alsD§

2

Alpha-acetolactate decarboxylase

BC0884

RZC01896

0.92

0.58

+

Ldh

 

L-lactate dehydrogenase

BC1924

RZC05932

0.44

1.05

-

lctP

 

L-lactate permease

BC0612

RZC01585

0.34

1.21

-

pta§

 

Phosphate acetyltransferase

BC5387

RZC05214

0.61

0.70

1

ackA§

 

Acetate kinase

BC4637

RZC02436

0.93

1.29

1

Citric acid cycle

citZ§

1

Citrate synthase

BC4594

RZC01265

3.76

2.23

1

citC§

2

Isocitrate dehydrogenase [NADP]

BC4593

RZC01263

5.56

4.38

1

mdh§

3

Malate dehydrogenase

BC4592

RZC01264

5.81

4.32

+

citB§

 

Aconitate hydratase

BC3616

RZC04993

1.62

1.26

1

odhA§

1

Oxoglutarate dehydrogenase

BC1252

RZC06639

2.07

3.80

4

odhB§

2

Dihydrolipoyllysine-residue succinyltransferase

BC1251

RZC03695

2.36

3.97

+

sucC§

1

Succinate-CoA ligase (ADP-forming)

BC3834

RZC02282

8.23

6.32

1

sucD§

2

Succinate-CoA ligase (ADP-forming)

BC3833

RZC02281

7.74

7.57

+

sdhC§

1

Succinate dehydrogenase

BC4518

RZC05750

4.02

4.02

1

sdhA§

2

Succinate dehydrogenase

BC4517

RZC07054

3.70

3.70

1

sdhB§

3

Succinate dehydrogenase

BC4516

RZC07053

3.76

5.64

+

citG§

 

Fumarate hydratase

BC1712

RZC03022

1.29

1.49

2

Gluconeogenensis

fbpA

 

Fructose-bisphosphatase

BC4962

RZC03080

0.75

0.74

2

gapB

 

Glyceraldehyde-3-P dehydrogenase

BC4583

RZC07229

1.74

2.79

3

pckA

 

Phosphoenolpyruvate carboxykinase (ATP)

BC4762

RZC07251

1.37

1.09

1

ywjI

 

Fructose-bisphosphatase

BC5333

RZC00125

3.73

3.34

+

  1. § Genes of which expression of their homologous gene in B. subtilis was CcpA dependent [18,19,21].
  2. # Genes of which effects of ccpA deletion on expression were shown to be indirect in B. subtilis [18, 19, 21].
  3. * pgk is represented on the microarray by two different amplicons, as Pgk is annotated in the ERGO database to be encoded by two genes.
  4. ¶ Numbers indicate the order of genes within one operon.
  5. † Ratios are presented as expression in the ccpA deletion strain compared to the wild-type (WT), expression in the wild type is set to 1. Early- and mid-exponential phase data are shown.
  6. ‡ In this column it is indicated whether a putative CRE-site was identified for the gene. Numbers indicate the number of putative CRE-sites identified, a + indicates that a gene is in the same operon as a gene for which a putative CRE-site was identified, and a - indicates no putative CRE-site could be identified.
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BMC Microbiology

ISSN: 1471-2180

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