Strains | Description | Source |
---|---|---|
Escherichia coli W3110 | F- prototroph | F.C. Neidhardt |
   E. coli BE10.2 | W3110 Δlac-169 lrp35::Tn10 | R.G. Matthews |
   E. coli BE3780 | lrp::Tn10, Δlac-169, gltB(psiQ35)::lacZ | R.G. Matthews |
   E. coli EPI300 | F- mcrA Δ (mrr-hsdRMS-mcrBC) Φ80d lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara, leu)7697 galU galK λ- rpsL nupG trfA dhfr | Epicentre |
   PS2209 | W3110 Δlac-169 | F. C. Neidhardt |
   MG1655 | F-lambda-ilvG-rfb-50 rph-1 | ATCC 700926 |
   MG1655 Δlrp | F-lambda-ilvG-rfb-50 rph-1 Δlrp::kan | This work |
Proteus mirabilis HI4320 | WT | H.L. Mobley |
   P. mirabilis U6450 | WT | G.M. Fraser |
   P. mirabilis U6450Δlrp | lrp::Tn5 | G.M. Fraser |
Vibrio cholerae El tor A1552 | WT | G.K. Schoolnik |
V. cholerae El tor A1552Δlrp | lrp-cat derivative of strain A1552 | G.K. Schoolnik |
Plasmids | Â | Â |
pCC1 | Low copy blunt cloning vector | Epicentre |
pECLRP | pCC1 backbone with lrp gene from E. coli O157:H7 inserted at BamHI site | This work |
pPMLRP | pCC1 backbone with lrp gene from P. mirabilis HI4320 inserted at BamHI site | This work |
pVCLRP | pCC1 backbone with lrp gene from V. cholerae El tor A1552 inserted at BamHI | This work |
pVEC | pCC1 backbone with Kan cassette cloned into BamHI site; used as vector control. | This work |
pRLIV2 | pACYC backbone with livK-lacZ transcriptional fusion. CAT gene was inactivated by digesting with NcoI, in-fill with Klenow fragment and religated to create a frame shift | This work |
pECKAN | pECLRP with CAT gene deleted and KAN cassette inserted at BsmI | This work |
pPMKAN | pPMLRP with CAT gene deleted and KAN cassette inserted at BsmI | This work |
pVCKAN | pVCLRP with CAT gene deleted and KAN cassette inserted at BsmI | This work |
pVEC2 | pVEC with CAT gene deleted and KAN cassette inserted at BsmI | This work |
pPM2005 | pBH403 backbone with E. coli O157 gltB promoter cloned at BamHI to SalI | [93] |
pPM2007 | pBH403 backbone with Proteus mirabilis gltB promoter cloned at BamHI to SalI | This work |
pPM2008 | pBH403 backbone with Vibrio cholerae Vc2376gltB-2 promoter cloned at BamHI to SalI | This work |
pPM2009 | pBH403 backbone with Vibrio cholerae Vc2373gltB-1 promoter cloned at BamHI to SalI | This work |
pPM3001 | pBH403 backbone with E. coli W3110 lrp promoter region cloned at BamHI to SalI | This work |
pPM3003 | pBH403 backbone with Proteus mirabilis lrp promoter cloned at BamHI to SalI | This work |
pPM3006 | pBH403 with Vibrio cholerae lrp promoter cloned at BamHI to SalI | This work |
pPM3001Chl | pPM3001 CAT gene was inactivated by digesting with with NcoI, in-fill with Klenow and religated to create a frame shift | This work |