Skip to main content

Table 1 Bacterial strains and plasmids used.

From: Limited functional conservation of a global regulator among related bacterial genera: Lrp in Escherichia, Proteus and Vibrio

Strains

Description

Source

Escherichia coli W3110

F- prototroph

F.C. Neidhardt

   E. coli BE10.2

W3110 Δlac-169 lrp35::Tn10

R.G. Matthews

   E. coli BE3780

lrp::Tn10, Δlac-169, gltB(psiQ35)::lacZ

R.G. Matthews

   E. coli EPI300

F- mcrA Δ (mrr-hsdRMS-mcrBC) Φ80d lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara, leu)7697 galU galK λ- rpsL nupG trfA dhfr

Epicentre

   PS2209

W3110 Δlac-169

F. C. Neidhardt

   MG1655

F-lambda-ilvG-rfb-50 rph-1

ATCC 700926

   MG1655 Δlrp

F-lambda-ilvG-rfb-50 rph-1 Δlrp::kan

This work

Proteus mirabilis HI4320

WT

H.L. Mobley

   P. mirabilis U6450

WT

G.M. Fraser

   P. mirabilis U6450Δlrp

lrp::Tn5

G.M. Fraser

Vibrio cholerae El tor A1552

WT

G.K. Schoolnik

V. cholerae El tor A1552Δlrp

lrp-cat derivative of strain A1552

G.K. Schoolnik

Plasmids

  

pCC1

Low copy blunt cloning vector

Epicentre

pECLRP

pCC1 backbone with lrp gene from E. coli O157:H7 inserted at BamHI site

This work

pPMLRP

pCC1 backbone with lrp gene from P. mirabilis HI4320 inserted at BamHI site

This work

pVCLRP

pCC1 backbone with lrp gene from V. cholerae El tor A1552 inserted at BamHI

This work

pVEC

pCC1 backbone with Kan cassette cloned into BamHI site; used as vector control.

This work

pRLIV2

pACYC backbone with livK-lacZ transcriptional fusion. CAT gene was inactivated by digesting with NcoI, in-fill with Klenow fragment and religated to create a frame shift

This work

pECKAN

pECLRP with CAT gene deleted and KAN cassette inserted at BsmI

This work

pPMKAN

pPMLRP with CAT gene deleted and KAN cassette inserted at BsmI

This work

pVCKAN

pVCLRP with CAT gene deleted and KAN cassette inserted at BsmI

This work

pVEC2

pVEC with CAT gene deleted and KAN cassette inserted at BsmI

This work

pPM2005

pBH403 backbone with E. coli O157 gltB promoter cloned at BamHI to SalI

[93]

pPM2007

pBH403 backbone with Proteus mirabilis gltB promoter cloned at BamHI to SalI

This work

pPM2008

pBH403 backbone with Vibrio cholerae Vc2376gltB-2 promoter cloned at BamHI to SalI

This work

pPM2009

pBH403 backbone with Vibrio cholerae Vc2373gltB-1 promoter cloned at BamHI to SalI

This work

pPM3001

pBH403 backbone with E. coli W3110 lrp promoter region cloned at BamHI to SalI

This work

pPM3003

pBH403 backbone with Proteus mirabilis lrp promoter cloned at BamHI to SalI

This work

pPM3006

pBH403 with Vibrio cholerae lrp promoter cloned at BamHI to SalI

This work

pPM3001Chl

pPM3001 CAT gene was inactivated by digesting with with NcoI, in-fill with Klenow and religated to create a frame shift

This work