Figure 4
From: OppA, the ecto-ATPase of Mycoplasma hominisinduces ATP release and cell death in HeLa cells
TaqMan PCR to quantify HeLa cell counts. The HeLa cell counts of an infection assay after incubation with M. hominis (Mhom), membrane proteins with (mem+) or without OppA (mem-), purified OppA and OppAΔP-loop-mutant were calculated by quantifying the copy numbers of the GAPDH-gene using real-time PCR.
