Figure 4From: OppA, the ecto-ATPase of Mycoplasma hominisinduces ATP release and cell death in HeLa cellsTaqMan PCR to quantify HeLa cell counts. The HeLa cell counts of an infection assay after incubation with M. hominis (Mhom), membrane proteins with (mem+) or without OppA (mem-), purified OppA and OppAΔP-loop-mutant were calculated by quantifying the copy numbers of the GAPDH-gene using real-time PCR.Back to article page