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Table 2 Oligonucleotide primers used in this study

From: Identification of critical residues in Gap3 of Streptococcus parasanguinis involved in Fap1 glycosylation, fimbrial formation and in vitroadhesion

Primer Sequence Reference or source
gap3-gfp-F 5'-CGGCCGTCGACATGACTAAACAGTTAATTTC-3' This study
gap3-gfp-R 5'-CGCCGCGGTACCAATATATTCTATTAAATTTTTC-3' This study
Δ62–67 Upstream 5'-GCAGACTCGAGAGAATGACGATCACCTTG-3' This study
Δ62–67 Downstream 5'-CGGGCCTCGAGATACAAGGAAATAGATACCTTC-3' This study
Δ144–157 Upstream 5'-GCCCGCCCTCGAGATCATAAATCATAAATTTTTG-3' This study
Δ144–157 Downstream 5'-TATCTCTCGAGTATCTACGGGAACTGCTTCCG-3' This study
Val33Arg-F 5'-CCAAGAGGATTTAAGGAATTTTTACAATCCACAGCTCGC-3' This study
Val33Arg-R 5'-GCGAGCTGTGGATTGTAAAAATTCCTTAAATCCTCTTGG-3' This study
Phe35His-F 5'-CCAAGAGGATTTAGTGAATCATTACAATCCACAGCTCGC-3' This study
Phe35His-R 5'-GCGAGCTGTGGATTGTAATGATTCACTAAATCCTCTTGG-3' This study
Asn37Ile-F 5'-GGATTTAGTGAATTTTTACATTCCACAGCTCGCATCAGGG-3' This study
Asn37Ile-R 5'-CCCTGATGCGAGCTGTGGAATGTAAAAATTCACTAAATCC-3' This study
Pro38Arg-F 5'-GTGAATTTTTACAATCGACAGCTCGCATCAGG-3' This study
Pro38Arg-R 5'-CCTGATGCGAGCTGTCGATTGTAAAAATTCAC-3' This study
Ser42Leu-F 5'-CAATCCACAGCTCGCATTAGGGAAGCCTATTG-3' This study
Ser42Leu-R 5'-CAATAGGCTTCCCTAATGCGAGCTGTGGATTG-3' This study
Asn54 Ile-F 5'-GTCACGAACAATTTATCAAGGTGATCGTCATTCTCC-3' This study
Asn54 Ile-R 5'-GGAGAATGACGATCACCTTGATAAATTGTTCGTGAC-3' This study
Pro62Gly-F 5'-GGTGATCGTCATTCTGGAGATCTTCCAATTC-3' This study
Pro62Gly-R 5'-GAATTGGAAGATCTCCAGAATGACGATCACC-3' This study
Asp63Val-F 5'-GGTGAT CGTCATTCTCCAGTTCTTCCAATTCTTATACAAGG-3' This study
Asp63Val-R 5'-CCTTGTATAAGAATTGGAAGAACTGGAGAATGACG ATCACC-3' This study
Leu64Arg-F 5'-CGTCATTCTCCAGATCGTCCAATTCTTATACAAGG-3' This study
Leu64Arg-R 5'-CCTTGTATAAGAATTGGACGATCTGGAGAATGACG-3' This study
Pro65Arg-F 5'-CGTCATTCTCCAGATCTTCGAATTCTTATACAAGG-3' This study
Pro65Arg-R 5'-CCTTGTATAAGAATTCGAAGATCTGGAGAATGACG-3' This study
Ile66Asn-F 5'-CGTCATTCTCCAGATCTTCCAAATCTTATACAAGG AAATAG-3' This study
Ile66Asn-R 5'-CTATTT CCTTGTATAAGATTTGGAAGATCTGGAGAATGACG-3' This study
Leu67Thr-F 5'-CGTCATTCTCCAGATCTTCCAATTACGATACAAGGAAATAG-3' This study
Leu67Thr-R 5'-CTATTTCCTTGTATCGTAATTGGAAGATCTGGAGAATGACG-3' This study
Leu75Ser-F 5'-GGAAATAGATACCTTTCAGAGTATCAGATTGATGCTGTACC-3' This study
Leu75Ser-R 5'-GGTACAGCATCAATCTGATACTCTGAAAGGTATCTATTTCC-3' This study
Arg59Leu-F 5'-CGAACAAATTATCAAGGTGATCTTCATTCTCCAGATCTTCC-3' This study
Arg59Leu-R 5'-GGAAGATCTGGAGAATGAAGATCACCTTGATAATTTGTTCG-3' This study