Table 2 Oligonucleotide primers used in this study
Primer | Sequence | Reference or source |
|---|---|---|
gap3-gfp-F | 5'-CGGCCGTCGACATGACTAAACAGTTAATTTC-3' | This study |
gap3-gfp-R | 5'-CGCCGCGGTACCAATATATTCTATTAAATTTTTC-3' | This study |
Δ62–67 Upstream | 5'-GCAGACTCGAGAGAATGACGATCACCTTG-3' | This study |
Δ62–67 Downstream | 5'-CGGGCCTCGAGATACAAGGAAATAGATACCTTC-3' | This study |
Δ144–157 Upstream | 5'-GCCCGCCCTCGAGATCATAAATCATAAATTTTTG-3' | This study |
Δ144–157 Downstream | 5'-TATCTCTCGAGTATCTACGGGAACTGCTTCCG-3' | This study |
Val33Arg-F | 5'-CCAAGAGGATTTAAGGAATTTTTACAATCCACAGCTCGC-3' | This study |
Val33Arg-R | 5'-GCGAGCTGTGGATTGTAAAAATTCCTTAAATCCTCTTGG-3' | This study |
Phe35His-F | 5'-CCAAGAGGATTTAGTGAATCATTACAATCCACAGCTCGC-3' | This study |
Phe35His-R | 5'-GCGAGCTGTGGATTGTAATGATTCACTAAATCCTCTTGG-3' | This study |
Asn37Ile-F | 5'-GGATTTAGTGAATTTTTACATTCCACAGCTCGCATCAGGG-3' | This study |
Asn37Ile-R | 5'-CCCTGATGCGAGCTGTGGAATGTAAAAATTCACTAAATCC-3' | This study |
Pro38Arg-F | 5'-GTGAATTTTTACAATCGACAGCTCGCATCAGG-3' | This study |
Pro38Arg-R | 5'-CCTGATGCGAGCTGTCGATTGTAAAAATTCAC-3' | This study |
Ser42Leu-F | 5'-CAATCCACAGCTCGCATTAGGGAAGCCTATTG-3' | This study |
Ser42Leu-R | 5'-CAATAGGCTTCCCTAATGCGAGCTGTGGATTG-3' | This study |
Asn54 Ile-F | 5'-GTCACGAACAATTTATCAAGGTGATCGTCATTCTCC-3' | This study |
Asn54 Ile-R | 5'-GGAGAATGACGATCACCTTGATAAATTGTTCGTGAC-3' | This study |
Pro62Gly-F | 5'-GGTGATCGTCATTCTGGAGATCTTCCAATTC-3' | This study |
Pro62Gly-R | 5'-GAATTGGAAGATCTCCAGAATGACGATCACC-3' | This study |
Asp63Val-F | 5'-GGTGAT CGTCATTCTCCAGTTCTTCCAATTCTTATACAAGG-3' | This study |
Asp63Val-R | 5'-CCTTGTATAAGAATTGGAAGAACTGGAGAATGACG ATCACC-3' | This study |
Leu64Arg-F | 5'-CGTCATTCTCCAGATCGTCCAATTCTTATACAAGG-3' | This study |
Leu64Arg-R | 5'-CCTTGTATAAGAATTGGACGATCTGGAGAATGACG-3' | This study |
Pro65Arg-F | 5'-CGTCATTCTCCAGATCTTCGAATTCTTATACAAGG-3' | This study |
Pro65Arg-R | 5'-CCTTGTATAAGAATTCGAAGATCTGGAGAATGACG-3' | This study |
Ile66Asn-F | 5'-CGTCATTCTCCAGATCTTCCAAATCTTATACAAGG AAATAG-3' | This study |
Ile66Asn-R | 5'-CTATTT CCTTGTATAAGATTTGGAAGATCTGGAGAATGACG-3' | This study |
Leu67Thr-F | 5'-CGTCATTCTCCAGATCTTCCAATTACGATACAAGGAAATAG-3' | This study |
Leu67Thr-R | 5'-CTATTTCCTTGTATCGTAATTGGAAGATCTGGAGAATGACG-3' | This study |
Leu75Ser-F | 5'-GGAAATAGATACCTTTCAGAGTATCAGATTGATGCTGTACC-3' | This study |
Leu75Ser-R | 5'-GGTACAGCATCAATCTGATACTCTGAAAGGTATCTATTTCC-3' | This study |
Arg59Leu-F | 5'-CGAACAAATTATCAAGGTGATCTTCATTCTCCAGATCTTCC-3' | This study |
Arg59Leu-R | 5'-GGAAGATCTGGAGAATGAAGATCACCTTGATAATTTGTTCG-3' | This study |