Skip to main content

Table 1 Bacterial strains, plasmids and antibodies used in this study

From: Identification of critical residues in Gap3 of Streptococcus parasanguinis involved in Fap1 glycosylation, fimbrial formation and in vitroadhesion

Strain, plasmid or antibody Relevant characteristics Reference or source
Strains   
   S. parasanguinis   
FW213 Wild type [7]
VT1393 fap1::aphA-3, null mutant of fap1, KanR [8]
VT1619 gap3 replacement mutant, KanR [23]
VT1731 pVT1666 into VT1619, vector control strain, ErmR & KanR This study
VT1732 pVT1732 into VT1619, gap3 complemented strain, ErmR &KanR This study
VT1735 Gap3 aa 62–67 encoding region deletion in pVT1732, ErmR &KanR This study
VT1737 Gap3 aa 144–157 encoding region deletion in pVT1732, ErmR &KanR This study
VT1738 Gap3 V33R mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1739 Gap3 F35F mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1740 Gap3 N37I mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1741 Gap3 P38R mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1742 Gap3 S42L mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1743 Gap3 N54I mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1744 Gap3 P62G mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1745 Gap3 D63V mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1746 Gap3 L64R mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1747 Gap3 P65R mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1748 Gap3 I66N mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1749 Gap3 L67T mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1750 Gap3 L75S mutation of pVT1732 in VT1619, ErmR &KanR This study
VT1751 Gap3 R59L mutation of pVT1732 in VT1619, ErmR &KanR This study
   E. coli   
DH10B Host strain for cloning Invitrogen
XL-10-Gold Host strain for site-directed mutagenesis Stratagene
Plasmids   
   pVT1571 An E. coli and S. parasanguinis shuttle vector, parent of pVPT1686 [11]
   pVT1666 PVT1571 vector contained gfp gene This study
   pVT1732 pVT1666 contained gap3 gene This study
Antibodies   
   D10 Partilly glycosylated Fap1 glycan epitope-specific MAb [20]
   E42 Fap1 peptide epitope-specific MAb [20]
   F51 Mature Fap1 glycan epitope-specific MAb [20]
   Anti-GFP MAb against GFP tag Roche Company