Strain, plasmid or antibody | Relevant characteristics | Reference or source |
---|---|---|
Strains | Â | Â |
   S. parasanguinis |  |  |
FW213 | Wild type | [7] |
VT1393 | fap1::aphA-3, null mutant of fap1, KanR | [8] |
VT1619 | gap3 replacement mutant, KanR | [23] |
VT1731 | pVT1666 into VT1619, vector control strain, ErmR & KanR | This study |
VT1732 | pVT1732 into VT1619, gap3 complemented strain, ErmR &KanR | This study |
VT1735 | Gap3 aa 62–67 encoding region deletion in pVT1732, ErmR &KanR | This study |
VT1737 | Gap3 aa 144–157 encoding region deletion in pVT1732, ErmR &KanR | This study |
VT1738 | Gap3 V33R mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1739 | Gap3 F35F mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1740 | Gap3 N37I mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1741 | Gap3 P38R mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1742 | Gap3 S42L mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1743 | Gap3 N54I mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1744 | Gap3 P62G mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1745 | Gap3 D63V mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1746 | Gap3 L64R mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1747 | Gap3 P65R mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1748 | Gap3 I66N mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1749 | Gap3 L67T mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1750 | Gap3 L75S mutation of pVT1732 in VT1619, ErmR &KanR | This study |
VT1751 | Gap3 R59L mutation of pVT1732 in VT1619, ErmR &KanR | This study |
   E. coli |  |  |
DH10B | Host strain for cloning | Invitrogen |
XL-10-Gold | Host strain for site-directed mutagenesis | Stratagene |
Plasmids | Â | Â |
   pVT1571 | An E. coli and S. parasanguinis shuttle vector, parent of pVPT1686 | [11] |
   pVT1666 | PVT1571 vector contained gfp gene | This study |
   pVT1732 | pVT1666 contained gap3 gene | This study |
Antibodies | Â | Â |
   D10 | Partilly glycosylated Fap1 glycan epitope-specific MAb | [20] |
   E42 | Fap1 peptide epitope-specific MAb | [20] |
   F51 | Mature Fap1 glycan epitope-specific MAb | [20] |
   Anti-GFP | MAb against GFP tag | Roche Company |