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Table 1 Bacterial strains, plasmids and antibodies used in this study

From: Identification of critical residues in Gap3 of Streptococcus parasanguinis involved in Fap1 glycosylation, fimbrial formation and in vitroadhesion

Strain, plasmid or antibody

Relevant characteristics

Reference or source

Strains

  

   S. parasanguinis

  

FW213

Wild type

[7]

VT1393

fap1::aphA-3, null mutant of fap1, KanR

[8]

VT1619

gap3 replacement mutant, KanR

[23]

VT1731

pVT1666 into VT1619, vector control strain, ErmR & KanR

This study

VT1732

pVT1732 into VT1619, gap3 complemented strain, ErmR &KanR

This study

VT1735

Gap3 aa 62–67 encoding region deletion in pVT1732, ErmR &KanR

This study

VT1737

Gap3 aa 144–157 encoding region deletion in pVT1732, ErmR &KanR

This study

VT1738

Gap3 V33R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1739

Gap3 F35F mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1740

Gap3 N37I mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1741

Gap3 P38R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1742

Gap3 S42L mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1743

Gap3 N54I mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1744

Gap3 P62G mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1745

Gap3 D63V mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1746

Gap3 L64R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1747

Gap3 P65R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1748

Gap3 I66N mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1749

Gap3 L67T mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1750

Gap3 L75S mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1751

Gap3 R59L mutation of pVT1732 in VT1619, ErmR &KanR

This study

   E. coli

  

DH10B

Host strain for cloning

Invitrogen

XL-10-Gold

Host strain for site-directed mutagenesis

Stratagene

Plasmids

  

   pVT1571

An E. coli and S. parasanguinis shuttle vector, parent of pVPT1686

[11]

   pVT1666

PVT1571 vector contained gfp gene

This study

   pVT1732

pVT1666 contained gap3 gene

This study

Antibodies

  

   D10

Partilly glycosylated Fap1 glycan epitope-specific MAb

[20]

   E42

Fap1 peptide epitope-specific MAb

[20]

   F51

Mature Fap1 glycan epitope-specific MAb

[20]

   Anti-GFP

MAb against GFP tag

Roche Company