Table 3 Sequences of PCR primers used for RT-qPCR¶
parAB expression | ||||
|---|---|---|---|---|
Gene | Forward (5'→3') | Reverse (5'→3') | Amplicon (bp) | Coordinates (5', 3') |
parA (Mb) | aagtgttgcggacggtgattc | ggtcacgctcggcaagttc | 140 | +874, +1014 |
parB (Mb) | gcgtaagccgattcagatgcc | ccgagccgaactccaccac | 122 | +833, +954 |
parA (Ms) | acgacggccgcaccaagct | gtcgagatagctcagtgctcc | 177 | +754, +930 |
parB (Ms) | cgtaagccgatccagatgcca | tcgttctgggcgctcatcag | 171 | +882, +1052 |
Co-transcription | ||||
Region | Forward (5'→3') | Reverse (5'→3') | Amplicon (bp) | Coordinates (5', 3') |
orf60K-jag (Mb) | aatgcggcagccccaacag | tcggtggtgtcagcgtcg | 256 | -233, +23 |
jag-gidB (Mb) | ccagaacgccgagtcgttgtgc | gtccgaagatcgcagacgc | 204 | -164, +40 |
gidB-parA (Mb) | gcggttgatgtcagggtggtg | cgtcggtgtcggtggtgtc | 236 | -124, +112 |
parA-parB (Mb) | gcgttggagggtgtgtcg | ccctttctgcgtgacggc | 352 | -326, +26 |
orf60K-jag (Ms) | gctccgccaccgaactgac | gcgtccgcagcgagagtg | 187 | -184, +3 |
jag-gidB (Ms) | ttccgccgcctcaagcc | cacgccctgtcctttgttctg | 199 | -124, +75 |
gidB-parA (Ms) | atgctcccgatcaaaggc | cgaacccatgctcatctcc | 230 | -215, +15 |
parA-parB (Ms) | cctcgcagtgtgaaggtctcg | cggctgattcatgctcgtctcc | 212 | -200, +12 |