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Table 1 Plasmids used in this work

From: par genes in Mycobacterium bovis and Mycobacterium smegmatisare arranged in an operon transcribed from "SigGC" promoters

Plasmid Relevant features Reference or source
pFPV27 Kmr, shuttle vector for operon and gene fusion to gfp gene [46]
pD19B 261 bp PCR fragment from M. bovis BCG containing the upstream region of the gene jag This work
pJ1B 148 bp PCR fragment from M. bovis BCG containing part of the coding region of the orf60K This work
pA3B 114 bp PCR fragment from M. bovis BCG containing the upstream region of the gene jag This work
pB5B 205 bp PCR fragment from M. bovis BCG containing the upstream region of the gene gidB This work
pA15B 116 bp PCR fragment from M. bovis BCG containing the coding region of the gene jag This work
pB3B 113 bp PCR fragment from M. bovis BCG containing the upstream region of the gene gidB This work
pA2B 214 bp PCR fragment from M. bovis BCG containing the upstream region of the gene parA This work
pC5B 113 bp PCR fragment from M. bovis BCG containing part of the coding region of the gene parA This work
pE1B 229 bp PCR fragment from M. bovis BCG containing the upstream region of the gene parB This work
pJ3M 320 bp PCR fragment from M. smegmatis containing the upstream region of the gene jag This work
pD1M 159 bp PCR fragment from M. smegmatis containing the upstream region of the gene jag This work
pG2M 256 bp PCR fragment from M. smegmatis containing part of the coding region of the gene jag This work
pC18M 217 bp PCR fragment from M. smegmatis containing part of the coding region of the gene parA cloned in the direction of parA gene This work
pC11M 217 bp PCR fragment from M. smegmatis containing part of the coding region of the gene parA cloned in the reverse direction of parA gene This work
pA1M 120 bp PCR fragment from M. smegmatis containing part of the coding region of the gene gidB This work
pB1M 200 bp PCR fragment from M. smegmatis containing part of the coding region of the gene gidB This work
pB16M 475 bp PCR fragment from M. smegmatis containing the upstream region of the gene parB This work
pC1M 122 bp PCR fragment from M. smegmatis containing the upstream region of the gene parB This work