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Table 1 Plasmids used in this work

From: par genes in Mycobacterium bovis and Mycobacterium smegmatisare arranged in an operon transcribed from "SigGC" promoters

Plasmid

Relevant features

Reference or source

pFPV27

Kmr, shuttle vector for operon and gene fusion to gfp gene

[46]

pD19B

261 bp PCR fragment from M. bovis BCG containing the upstream region of the gene jag

This work

pJ1B

148 bp PCR fragment from M. bovis BCG containing part of the coding region of the orf60K

This work

pA3B

114 bp PCR fragment from M. bovis BCG containing the upstream region of the gene jag

This work

pB5B

205 bp PCR fragment from M. bovis BCG containing the upstream region of the gene gidB

This work

pA15B

116 bp PCR fragment from M. bovis BCG containing the coding region of the gene jag

This work

pB3B

113 bp PCR fragment from M. bovis BCG containing the upstream region of the gene gidB

This work

pA2B

214 bp PCR fragment from M. bovis BCG containing the upstream region of the gene parA

This work

pC5B

113 bp PCR fragment from M. bovis BCG containing part of the coding region of the gene parA

This work

pE1B

229 bp PCR fragment from M. bovis BCG containing the upstream region of the gene parB

This work

pJ3M

320 bp PCR fragment from M. smegmatis containing the upstream region of the gene jag

This work

pD1M

159 bp PCR fragment from M. smegmatis containing the upstream region of the gene jag

This work

pG2M

256 bp PCR fragment from M. smegmatis containing part of the coding region of the gene jag

This work

pC18M

217 bp PCR fragment from M. smegmatis containing part of the coding region of the gene parA cloned in the direction of parA gene

This work

pC11M

217 bp PCR fragment from M. smegmatis containing part of the coding region of the gene parA cloned in the reverse direction of parA gene

This work

pA1M

120 bp PCR fragment from M. smegmatis containing part of the coding region of the gene gidB

This work

pB1M

200 bp PCR fragment from M. smegmatis containing part of the coding region of the gene gidB

This work

pB16M

475 bp PCR fragment from M. smegmatis containing the upstream region of the gene parB

This work

pC1M

122 bp PCR fragment from M. smegmatis containing the upstream region of the gene parB

This work