Figure 3From: Novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of PatagoniaPhylogenetic analysis of the deduced amino acid sequences of ARHD genes found in the libraries. The tree was constructed by using the neighbor-joining method in MEGA 3.1 [57] using clone sequences and related proteins, corresponding to positions 45–192 of NahAc in P. putida NCIB 9816-4. Bootstrap values were calculated as percentage of 1000 replicates, and values greater than 50% are shown at branching points. The scale bar represents 0.1 inferred amino acid changes per position. Branches containing sequences found in this study are indicated with a rectangle, with shading indicating novel ARHD gene types. The sequence abbreviations (protein name and strain number), species and accession numbers from GenBank are as follows: BphA IAM 12620: Porphyrobacter sanguineus IAM 12620 [BAB55875]; PhnA1a CHY-1: Sphingomonas sp. CHY-1 [CAG17576]; BphA1f B1: Sphingomonas yanoikuyae B1 [ABM91740]; PhnA1 A5: Cycloclasticus sp. A5 [BAC81541]; PhnAc AFK2 type: includes PhnAc A. faecalis AFK2 [BAA76323], PhnAc Burkholderia sp. Eh1-1 [AAQ84686], PhnAc Burkholderia sp. Cs1-4 [AAQ84685], PhnAc Burkholderia sp. Ch3-5 [AAQ84684] and sequences from this work; ArhA1 A4: Sphingomonas sp. A4 [BAD34447]; PhnAc RP007: Burkholderia sp. RP007 [AAD09872]; NagAc type: includes NagAc Ralstonia sp. U2 [AAD12610], NbzAc Comamonas sp. JS765 [AAL76202], NtdAc Acidovorax sp. JS42 [AAB40383]; NahAc type: includes NahAc P. putida NCIB 9816-4 [AAO64274], NahAc P. putida G7 [BAE92156], NahAc Pseudomonas sp. ND6 [AAP44288] and sequences from this work; CarAa CB3: Sphingomonas sp. CB3 [AAC38616]; XylC1 RB1: Cycloclasticus oligotrophus RB1 [AAC44161]; BphAa RHA1: Rhodococcus sp. RHA1 [YP707265]; CumA1 IP01: Pseudomonas fluorescens IP01 [BAA07074]; BphA1 B4: Pseudomonas sp. B4 [CAB93965]; BphA LB400: Burkholderia sp. LB400 [AAB63425]; NidA PYR-1: Mycobacterium vanbaalenii PYR-1 [AAT51751]; PhtA1 DBF63: Terrabacter sp. DBF63 [BAC54156]; NarA NCIMB12038: Rhodococcus sp. NCIMB 12038 [AAD28100]; NidA I24: Rhodococcus sp. I24 [AAD25395]; PhdA KP7: Nocardioides sp. KP7 [BAA94708]; DitA BKME-9: Pseudomonas abietaniphila BKME-9 [AAD21063]; DbfA1 DBF63: Terrabacter sp. DBF63 [BAB55886]; XylX pWW0: P. putida pWW0 [AAA26047]; XylX B1: Sphingomonas yanoikuyae B1 [ABM79785]. The arrow corresponds to sequences CarAa CA10: Pseudomonas resinovorans CA10 [BAB32766] and Pht3 NMH102-2: Pseudomonas putida NMH102-2 [BAA02511].Back to article page