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Table 1 Parameter definitions and ranges used in our biomathematical simulations

From: Spatial constraints within the chlamydial host cell inclusion predict interrupted development and persistence

Parameter Definition Range References
t d Average doubling time of RBs during phase of exponential growth (replication and division of bodies) 1.5–2.6 hrs [1-3]
α 0 Proportion of total doubling time in which RB genomes are dividing into separate bodies during uninhibited exponential growth 0.1–0.5  
1/k Average time for RB detachment from the inclusion membrane and migration to the lumen to occur, when the number of injectisomes is half the threshold level 0.01–1.0 hrs
1/μ Average time for RB-EB differentiation 1–4 hrs [2, 3, 38]
L Average center-to-center spacing between T3S needles on the RB surface 40–50 nm [10, 32]
l p Average length of each needle 5–10 nm § [33]
r Radius of each RB (without stress) 0.5–0.6 μm [38]
r E Radius of each EB 0.1–0.2 μm [38]
Cell vol Volume of host cell 1700–2500 μm3 Experimental Variable
ε Proportion of cell space occupied by cell nucleus, mitochondria, etc. 0.2–0.4 Experimental Variable
V I Volume within the lumen occupied per detached RB (considering effects of steric hindrance) V I = (2r)3
V E Volume within the lumen occupied per EB (considering effects of steric hindrance) V E = (2r E )3
P lim Threshold number of injectisomes per RB for the detachment of RBs from the inclusion membrane 21–25 [9]
  1. †: Experimental variable. It was calibrated so that it matched observational experimental time courses.
  2. §: The full length of the needle has been measured to be 10–25 nm, but we assume that the length of the needle between the RB and the CIM required to establish effective contact is 5–10 nm.