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Table 4 Primer sequences and characteristics. Primers were used to generate PCR amplicons for uses as probes for Southern hybridizations or as template for DNA sequencing. Primers were based on the bacteriophage genes present in RM 1221 (Genbank accession number CP000025) and were selected with the aid of the PrimerSelect™ program in the Lasergene DNASTAR™ version 5.06 software. phig3r and phig4r primers were used with the phig2f primer to amplify regions that could not be amplified with the phig2f/phig2r combination.

From: Sequence variability of Campylobactertemperate bacteriophages

Primer Name Sequence 5' → 3' Annealing temperature (°C) Amplicon size Position in RM1221 genome
cje0215F GCG AGT GAA GGC AAA AG 47 351 bp 207652 – 207668
cje0215R TTC TCC ATA GCA AGT GAT AAA C    208002 – 207981
cje0221F ATT ATG GCG GGT GCT GGA G 45 330 bp 210639 – 210657
cje0221R GAC TTT GTT ATT ATC TAT GG    210328 – 210347
cje0226F TGG CGA AGT TAT ACA GGA AGG T 50 207 bp 214289 – 214310
cje0226R AAG AAA GCC GCA TAA AGC ACT    214104 – 204124
cje0232F TAA ACC ACC ATC CAA AAC AAA G 50 296 bp 219214 – 219235
cje0232R TCC GCC ATA ATT AAA CCA CTC    218940 – 218960
cje0244F TAC CGC TAT TTA TCC CCT GTG T 50 359 bp 223836 – 223857
cje0244R ATT AGC GCC CAT TCT TTT TG    224175 – 224194
cje0251F ATG GGG ATA AAT TTA GCA CTT G 50 392 bp 230266 – 230287
cje0251R TAG GCC TTT AAC TTC ACT TTC AC    230657 – 230636
cje0270F TTC ACC GCA AAG ATA AAA CTA A 50 373 bp 241603 – 241582
cje0270R ACT ATA ATA TCA GCT GGG GAA CTA    241231 – 241254
cje0544F AAT AGG GGA ATG CCA AAA A 45 357 bp 498853 – 498871
cje0544R CTA CTA ATC TCA AAT ATC CTA CAT    499209 – 499186
cje0569F ATT AAC TTC AGA TAT TTC CCA GAT 47 420 bp 513074 – 513097
cje0569R AAC AGC CAT TTT TGA TAC TAC AG    512678 – 512700
cje1418F ATC CGT TAC TTT CCT TAG CAG AGC 51 540 bp 1335107 – 1336130
cje1418R CAT TAC CGG GGC GTT GTG    1336629 – 1336646
cje1454F TAG TGG CTT ATC TTT AGT C 43 158 bp 1354180 – 1354198
cje1454R ATT TCC TTG CTT TTA TT    1354041 – 1354057
cje1471F CCG CAA ATG AAA CCG AAC AA 52 475 bp 1369607 – 1369626
cje1471R GCC ATA ACC CAA AGC AGG ATT    1369151 – 1369171
phs5f TGC TAG GCT TTG GGG TTT GTT 52 2679 bp 209776 – 209796
phs5r AGC GCT GAA GAT GTG GGA GAT AG    212432 – 212454
phs6f GTT TAG GCG GAG GCG GAA TA 51 2340 bp 207887 – 207906
phs6r GTC GGG CGT GGT CTT TAG TGA    210206 – 210226
pcc1f TAT GTG AAA TTA GTT GGC GAA GAT 51 3654 bp 208668 – 208691
pcc1r TTG TGA GCA GAA TTG GAG GAA    in 00–2425
phs0f GCC ATT CTT GCT AAC ACT TTT TGA 51 1559 bp 210155 – 210178
phs0r AAT GGG GTT TTA GGA GGA CTT AT    211691 – 211713
phs1f AAG GCT TTA AGG GAG GTT GTG T 52 2918 bp 211484 – 211505
phs1r TCT GAT GAA TGG GCG AGT GA    214382 – 214401
phig2f AGA AAG CCG CAT AAA GCA CT 52 1069 bp 214104 – 214124
phig2r AAA AGC AAA GCA CAA AAC AGG    215153 – 215173
phig3r AAA AGC CAA ACA TAA AAC AGG    
phig4r AAA AGC CAA GCA TAA AAG AGG    
phig1f GGC TTT AAA ACC CCG CTA CTA 51 814 bp 214161 – 214181
phig1r TGG CCA CAG GTT CAA ATC TTA    214954 – 214974
phs2f CCC ACC CCA AGA GCGATA AC 52 2783 bp 214711 – 214730
phs2r AGG ATG AAG TGA GCG ATG AGC A    217472 – 217493
phs3f AAC CCC CAT ATT GTC CAC 49 2863 bp 216133 – 216150
phs3r CTT TAA GAG CCG TAT TTC CTA    218975 – 218995