Table 4 Primer sequences and characteristics. Primers were used to generate PCR amplicons for uses as probes for Southern hybridizations or as template for DNA sequencing. Primers were based on the bacteriophage genes present in RM 1221 (Genbank accession number CP000025) and were selected with the aid of the PrimerSelectâ„¢ program in the Lasergene DNASTARâ„¢ version 5.06 software. phig3r and phig4r primers were used with the phig2f primer to amplify regions that could not be amplified with the phig2f/phig2r combination.
From: Sequence variability of Campylobactertemperate bacteriophages
Primer Name | Sequence 5' → 3' | Annealing temperature (°C) | Amplicon size | Position in RM1221 genome |
|---|---|---|---|---|
cje0215F | GCG AGT GAA GGC AAA AG | 47 | 351 bp | 207652 – 207668 |
cje0215R | TTC TCC ATA GCA AGT GAT AAA C |  |  | 208002 – 207981 |
cje0221F | ATT ATG GCG GGT GCT GGA G | 45 | 330 bp | 210639 – 210657 |
cje0221R | GAC TTT GTT ATT ATC TAT GG |  |  | 210328 – 210347 |
cje0226F | TGG CGA AGT TAT ACA GGA AGG T | 50 | 207 bp | 214289 – 214310 |
cje0226R | AAG AAA GCC GCA TAA AGC ACT |  |  | 214104 – 204124 |
cje0232F | TAA ACC ACC ATC CAA AAC AAA G | 50 | 296 bp | 219214 – 219235 |
cje0232R | TCC GCC ATA ATT AAA CCA CTC |  |  | 218940 – 218960 |
cje0244F | TAC CGC TAT TTA TCC CCT GTG T | 50 | 359 bp | 223836 – 223857 |
cje0244R | ATT AGC GCC CAT TCT TTT TG |  |  | 224175 – 224194 |
cje0251F | ATG GGG ATA AAT TTA GCA CTT G | 50 | 392 bp | 230266 – 230287 |
cje0251R | TAG GCC TTT AAC TTC ACT TTC AC |  |  | 230657 – 230636 |
cje0270F | TTC ACC GCA AAG ATA AAA CTA A | 50 | 373 bp | 241603 – 241582 |
cje0270R | ACT ATA ATA TCA GCT GGG GAA CTA |  |  | 241231 – 241254 |
cje0544F | AAT AGG GGA ATG CCA AAA A | 45 | 357 bp | 498853 – 498871 |
cje0544R | CTA CTA ATC TCA AAT ATC CTA CAT |  |  | 499209 – 499186 |
cje0569F | ATT AAC TTC AGA TAT TTC CCA GAT | 47 | 420 bp | 513074 – 513097 |
cje0569R | AAC AGC CAT TTT TGA TAC TAC AG |  |  | 512678 – 512700 |
cje1418F | ATC CGT TAC TTT CCT TAG CAG AGC | 51 | 540 bp | 1335107 – 1336130 |
cje1418R | CAT TAC CGG GGC GTT GTG |  |  | 1336629 – 1336646 |
cje1454F | TAG TGG CTT ATC TTT AGT C | 43 | 158 bp | 1354180 – 1354198 |
cje1454R | ATT TCC TTG CTT TTA TT |  |  | 1354041 – 1354057 |
cje1471F | CCG CAA ATG AAA CCG AAC AA | 52 | 475 bp | 1369607 – 1369626 |
cje1471R | GCC ATA ACC CAA AGC AGG ATT |  |  | 1369151 – 1369171 |
phs5f | TGC TAG GCT TTG GGG TTT GTT | 52 | 2679 bp | 209776 – 209796 |
phs5r | AGC GCT GAA GAT GTG GGA GAT AG |  |  | 212432 – 212454 |
phs6f | GTT TAG GCG GAG GCG GAA TA | 51 | 2340 bp | 207887 – 207906 |
phs6r | GTC GGG CGT GGT CTT TAG TGA |  |  | 210206 – 210226 |
pcc1f | TAT GTG AAA TTA GTT GGC GAA GAT | 51 | 3654 bp | 208668 – 208691 |
pcc1r | TTG TGA GCA GAA TTG GAG GAA |  |  | in 00–2425 |
phs0f | GCC ATT CTT GCT AAC ACT TTT TGA | 51 | 1559 bp | 210155 – 210178 |
phs0r | AAT GGG GTT TTA GGA GGA CTT AT |  |  | 211691 – 211713 |
phs1f | AAG GCT TTA AGG GAG GTT GTG T | 52 | 2918 bp | 211484 – 211505 |
phs1r | TCT GAT GAA TGG GCG AGT GA |  |  | 214382 – 214401 |
phig2f | AGA AAG CCG CAT AAA GCA CT | 52 | 1069 bp | 214104 – 214124 |
phig2r | AAA AGC AAA GCA CAA AAC AGG |  |  | 215153 – 215173 |
phig3r | AAA AGC CAA ACA TAA AAC AGG | Â | Â | Â |
phig4r | AAA AGC CAA GCA TAA AAG AGG | Â | Â | Â |
phig1f | GGC TTT AAA ACC CCG CTA CTA | 51 | 814 bp | 214161 – 214181 |
phig1r | TGG CCA CAG GTT CAA ATC TTA |  |  | 214954 – 214974 |
phs2f | CCC ACC CCA AGA GCGATA AC | 52 | 2783 bp | 214711 – 214730 |
phs2r | AGG ATG AAG TGA GCG ATG AGC A |  |  | 217472 – 217493 |
phs3f | AAC CCC CAT ATT GTC CAC | 49 | 2863 bp | 216133 – 216150 |
phs3r | CTT TAA GAG CCG TAT TTC CTA |  |  | 218975 – 218995 |