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Table 3 Results for 12 NTM isolates for which PRA-hsp65 gave incorrect species identification as determined by hsp65 sequencing.

From: Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65patterns

Species

Nb

PRA-hsp65result

M. avium (18)c

1

M. kansasii 1

M. farcinogenes (1)

1

M. scrofulaceum 1

M. intracellulare (9)

1

M. avium 3

M. kansasii (7)

1

M. avium 2

 

1

M. gastri 1

M. mucogenicum (8)

1

M. chitae 1

 

1

M. gordonae 1

 

1

M. nonchromogenicum 1

M. nebraskense (1)

1

M. avium 3

M. scrofulaceum (3)

2

M. lentiflavum 3

 

1

M. simiae 1

  1. a Species identification was determined by hsp65 sequencing for 113 isolates that had discordant results by PRA-hsp65 and phenotypic studies. For 71 isolates sequencing confirmed the species identification obtained by PRA-hsp65. For an additional 30 isolates, the PRA-hsp65 patterns obtained were previously unreported (see Table 4).
  2. b N, number of isolates for which the PRA-hsp65 identification shown was incorrect.
  3. c Total number of isolates of that species sequenced.