Table 1 TR339-eGFP Infection Profiles following dsRNA Injection. Significantly enhanced viral eGFP fluorescence occurs at 4 dpi in midguts following Ago2 dsRNA treatment and in disseminated tissues at 7 dpi following TSNdsRNA treatment.
From: Aedes aegyptiuses RNA interference in defense against Sindbis virus infection
| Â | Â | Day 4 | Day 7 | ||
|---|---|---|---|---|---|
dsRNA | Â | Midgut | Dissemination | Midgut | Dissemination |
βgal | Light | 28/47 (59.6%) |  | 38/76 (50%) |  |
| Â | Moderate | 2/47 (4.3%) | Â | 5/76 (6.6%) | Â |
| Â | Heavy | 3/47 (6.4%) | Â | 5/76 (6.6%) | Â |
| Â | Total | 33/47 (70.2%) | 10/33 (30%) | 48/76 (63.2%) | 12/48 (25%) |
Ago2 | Light | 7/25 (28%) | Â | 20/53 (37.7%) | Â |
| Â | Moderate | 6/25 (24%) | Â | 14/53 (26.4%) | Â |
| Â | Heavy | 12/25 (48%) | Â | 5/53 (9.4%) | Â |
| Â | Total | 25/25 (100%)** | 12/25 (48%) | 39/53 (73.5%) | 5/39 (12.8%) |
Dcr2 | Light | 9/20 (45%) | Â | 7/19 (36.8%) | Â |
| Â | Moderate | 2/20 (10%) | Â | 2/19 (10.5%) | Â |
| Â | Heavy | 0/20 (0%) | Â | 0/19 (0%) | Â |
| Â | Total | 11/20 (55%) | 5/11 (45.5%) | 9/19 (47.3%) | 3/9 (33.3%) |
TSN | Light | 12/31 (38.7%) | Â | 10/37 (27%) | Â |
| Â | Moderate | 4/31 (12.9%) | Â | 2/37 (5.4%) | Â |
| Â | Heavy | 0/31 (0%) | Â | 3/37 (8.1%) | Â |
| Â | Total | 16/31 (51.6%) | 5/16 (31.3%) | 15/37 (40.5%)^^ | 15/15 (100%)** |
nsP3 | Light | 1/32 (3.1%) | Â | 1/28 (3.6%) | Â |
| Â | Moderate | 0/1 (0%) | Â | 0/28 (0%) | Â |
| Â | Heavy | 0/32 (0%) | Â | 0/28 (0%) | Â |
| Â | Total | 1/32 (3.1%)^^ | 0/1 (0%) | 1/28 (3.6%)^^ | 0/1 (0%) |