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Table 1 TR339-eGFP Infection Profiles following dsRNA Injection. Significantly enhanced viral eGFP fluorescence occurs at 4 dpi in midguts following Ago2 dsRNA treatment and in disseminated tissues at 7 dpi following TSNdsRNA treatment.

From: Aedes aegyptiuses RNA interference in defense against Sindbis virus infection

   Day 4 Day 7
dsRNA   Midgut Dissemination Midgut Dissemination
βgal Light 28/47 (59.6%)   38/76 (50%)  
  Moderate 2/47 (4.3%)   5/76 (6.6%)  
  Heavy 3/47 (6.4%)   5/76 (6.6%)  
  Total 33/47 (70.2%) 10/33 (30%) 48/76 (63.2%) 12/48 (25%)
Ago2 Light 7/25 (28%)   20/53 (37.7%)  
  Moderate 6/25 (24%)   14/53 (26.4%)  
  Heavy 12/25 (48%)   5/53 (9.4%)  
  Total 25/25 (100%)** 12/25 (48%) 39/53 (73.5%) 5/39 (12.8%)
Dcr2 Light 9/20 (45%)   7/19 (36.8%)  
  Moderate 2/20 (10%)   2/19 (10.5%)  
  Heavy 0/20 (0%)   0/19 (0%)  
  Total 11/20 (55%) 5/11 (45.5%) 9/19 (47.3%) 3/9 (33.3%)
TSN Light 12/31 (38.7%)   10/37 (27%)  
  Moderate 4/31 (12.9%)   2/37 (5.4%)  
  Heavy 0/31 (0%)   3/37 (8.1%)  
  Total 16/31 (51.6%) 5/16 (31.3%) 15/37 (40.5%)^^ 15/15 (100%)**
nsP3 Light 1/32 (3.1%)   1/28 (3.6%)  
  Moderate 0/1 (0%)   0/28 (0%)  
  Heavy 0/32 (0%)   0/28 (0%)  
  Total 1/32 (3.1%)^^ 0/1 (0%) 1/28 (3.6%)^^ 0/1 (0%)
  1. "()" indicates percentage eGFP detected in each group.
  2. "**" indicates infection rate higher than βGAL controls. "^^" indicates infectious rate significantly lower than βGAL controls.
  3. Light: 1–33% of the midgut infected; Moderate: 34–66%; Heavy: 67–100%; illustrations of infection patterns have been previously reported [33, 35].