Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia

Table 2 Diversity of the Bcc collection examined and results of repA multiplex PCR.

Bcc species/group	No. isolates	No. STs	636 bp product	237 bp product
B. cepacia	9	7	+	-
B. multivorans	15	14	-	-
B. cenocepacia IIIA	13	11	+	+
B. cenocepacia IIIB	29	27	+	+
B. cenocepacia IIIC	3	3	-	-
B. cenocepacia IIID	2	1	+	+
B. stabilis	5	3	+	-
B. vietnamiensis	10	6	+ (1 ST negative)	-
B. dolosa	3	3	+	-
B. ambifaria	18	11	+ (1 ST negative)	-
B. anthina	7	7	+	-
B. pyrrocinia	5	5	+ (1 ST negative)	-
Bcc Group E	3	3	+	-
Bcc Group K	8	6	+	-
Bcc2	2	1	+	-
Bcc3	3	3	+	-
Bcc4	3	3	+	-
Bcc5	12	9	-	- (3 STs positive)
Bcc6	19	18	+	- (7 STs positive)
Bcc8	1	1	+	-
Total	170	142

The number of isolates and STs for each Bcc group or species is shown. Certain STs within these groups produced the opposite repA multiplex PCR result to that expected for either the 636 bp or 237 bp product; the number of STs and the PCR result for these non-conforming strains are indicated in brackets.

ISSN: 1471-2180