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Table 1 Primers used in this study.

From: Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia

Primer Purpose Sequence (5' to 3') a,b Product size (bp)
repA-UNI-5 universal repA PCR GGA TGT GGT GAG TGC CAG TTC A 898
repA-UNI-3 universal repA PCR CCG CTG YTC GGT CAT CTG C  
repA-SEQ-5 repA sequencing species-specific PCR CAG CAG GCC GAC GAC TCG 623
repA-SEQ-3 repA sequencing ATC GGC TGC TTG CGY TCG GT  
repA-636-3 species-specific PCR GTC GAG CGC GAG CAT CGG C 636
repA-237-3 species-specific PCR CCA CAC GCG GCG GGT GGT 237
parB-UNI-5 universal parB PCR
parB sequencing
GCA GCA GGC GAT YCA CGT G 656
parB-UNI-3 universal parB PCR
parB sequencing
CGT CGC GCT TST CCT TCG G  
  1. a Standard code, Y = C or T; S = C or G
  2. b Mis-matched nucleotide positions in the species specific primers are underlined