Skip to main content

Table 1 Primers used in this study.

From: Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia

Primer

Purpose

Sequence (5' to 3') a,b

Product size (bp)

repA-UNI-5

universal repA PCR

GGA TGT GGT GAG TGC CAG TTC A

898

repA-UNI-3

universal repA PCR

CCG CTG YTC GGT CAT CTG C

 

repA-SEQ-5

repA sequencing species-specific PCR

CAG CAG GCC GAC GAC TCG

623

repA-SEQ-3

repA sequencing

ATC GGC TGC TTG CGY TCG GT

 

repA-636-3

species-specific PCR

GTC GAG CGC GAG CAT CGG C

636

repA-237-3

species-specific PCR

CCA CAC GCG GCG GGT GGT

237

parB-UNI-5

universal parB PCR

parB sequencing

GCA GCA GGC GAT YCA CGT G

656

parB-UNI-3

universal parB PCR

parB sequencing

CGT CGC GCT TST CCT TCG G

 
  1. a Standard code, Y = C or T; S = C or G
  2. b Mis-matched nucleotide positions in the species specific primers are underlined