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Table 8 Results obtained for the pressure ulcer sample (P) using shotgun Sanger sequencing (FRACS).

From: Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

Genus species Seq % Gram Aerotolerance Shape
Peptoniphilus ivorii 51 27.42 + anaerobic cocci
Anaerococcus lactolyticus 20 10.75 + anaerobic cocci
Anaerococcus vaginalis 16 8.60 + anaerobic cocci
Streptococcus dysgalactiae* 14 7.53 + facultative cocci
Peptoniphilus harei 14 7.53 + anaerobic cocci
Peptococcus niger 12 6.45 + anaerobic rod
Serratia marcescens 12 6.45 - aerobic rod
Finegoldia magna 10 5.38 + anaerobic cocci
Peptoniphilus indolicus 7 3.76 + anaerobic cocci
Clostridium hathewayi 4 2.15 + anaerobic rod
Prevotella buccalis 3 1.61 - anaerobic rod
Pseudomonas aeruginosa* 3 1.61 - aerobic rod
UWB 2 1.08 Unk unk unk
Dialister invisus 2 1.08 - anaerobic rod
Dialister spp. 2 1.08 - anaerobic rod
Delftia acidovorans 2 1.08 - aerobic rod
Pseudomonas fluorescens* 2 1.08 - aerobic rod
Helcococcus kunzii 1 0.54 + facultative anaerobe cocci
Enterococcus faecalis* 1 0.54 + facultative cocci
Staphylococcus epidermidis* 1 0.54 + facultative cocci
Streptococcus pyogenes* 1 0.54 + facultative cocci
Clostridium perfringens 1 0.54 + anaerobic rod
Klebsiella granulomatis 1 0.54 - Facultative anaerobe rod
Klebsiella pneumoniae 1 0.54 - Facultative anaerobe rod
Proteus vulgaris* 1 0.54 - Facultative anaerobe rod
Porphyromonas uenonis 1 0.54 - anaerobic rod
Pseudomonas spp.* 1 0.54 - aerobic rod
  1. This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the P sample, the number of sequences corresponding to the given genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using FRACSS include Acinetobacter, Enterobacter, Leclercia and Morganella. The % represents the percentage of the total sequences analyzed within the sample.