Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

BMC Microbiology

Table 8 Results obtained for the pressure ulcer sample (P) using shotgun Sanger sequencing (FRACS).

Genus species	Seq	%	Gram	Aerotolerance	Shape
Peptoniphilus ivorii	51	27.42	+	anaerobic	cocci
Anaerococcus lactolyticus	20	10.75	+	anaerobic	cocci
Anaerococcus vaginalis	16	8.60	+	anaerobic	cocci
Streptococcus dysgalactiae*	14	7.53	+	facultative	cocci
Peptoniphilus harei	14	7.53	+	anaerobic	cocci
Peptococcus niger	12	6.45	+	anaerobic	rod
Serratia marcescens	12	6.45	-	aerobic	rod
Finegoldia magna	10	5.38	+	anaerobic	cocci
Peptoniphilus indolicus	7	3.76	+	anaerobic	cocci
Clostridium hathewayi	4	2.15	+	anaerobic	rod
Prevotella buccalis	3	1.61	-	anaerobic	rod
Pseudomonas aeruginosa*	3	1.61	-	aerobic	rod
UWB	2	1.08	Unk	unk	unk
Dialister invisus	2	1.08	-	anaerobic	rod
Dialister spp.	2	1.08	-	anaerobic	rod
Delftia acidovorans	2	1.08	-	aerobic	rod
Pseudomonas fluorescens*	2	1.08	-	aerobic	rod
Helcococcus kunzii	1	0.54	+	facultative anaerobe	cocci
Enterococcus faecalis*	1	0.54	+	facultative	cocci
Staphylococcus epidermidis*	1	0.54	+	facultative	cocci
Streptococcus pyogenes*	1	0.54	+	facultative	cocci
Clostridium perfringens	1	0.54	+	anaerobic	rod
Klebsiella granulomatis	1	0.54	-	Facultative anaerobe	rod
Klebsiella pneumoniae	1	0.54	-	Facultative anaerobe	rod
Proteus vulgaris*	1	0.54	-	Facultative anaerobe	rod
Porphyromonas uenonis	1	0.54	-	anaerobic	rod
Pseudomonas spp.*	1	0.54	-	aerobic	rod

This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the P sample, the number of sequences corresponding to the given genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using FRACSS include Acinetobacter, Enterobacter, Leclercia and Morganella. The % represents the percentage of the total sequences analyzed within the sample.

ISSN: 1471-2180