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Table 7 Results obtained for diabetic foot ulcer sample (D) using shotgun Sanger sequencing (FRACS).

From: Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

Genus species Seq % Gram Aerotolerance Shape
Staphylococcus aureus * 70 39.33 + facultative Cocci
Anaerococcus lactolyticus 16 8.99 + anaerobic Cocci
Anaerococcus vaginalis 15 8.43 + anaerobic cocci
Bacterioides fragilis 7 3.93 - anaerobic rod
Finegoldia magna 6 3.37 + anaerobic cocci
Morganella morganii 5 2.81 - Facultative anaerobe rod
Enterococcus faecalis* 5 2.81 + facultative cocci
Peptoniphilus harei 5 2.81 + anaerobic cocci
Clostridium novyi 4 2.25 + anaerobic rod
Veillonella atypica 4 2.25 - anaerobic cocci
Abiotrophia para-adiacens 4 2.25 + anaerobic cocci
Veillonella parvula 4 2.25 - anaerobic cocci
Citrobacter murliniae * 3 1.69 - facultative anaerobe rod
Haemophilus spp. 3 1.69 - facultative rod
Clostridium spp. 3 1.69 + anaerobic rod
Haemophilus segnis 3 1.69 - faculative rod
Enterococcus avium* 3 1.69 + facultative cocci
Proteus spp.* 2 1.12 - Facultative anaerobe rod
Enterobacter aerogenes 2 1.12 - facultative rod
Dialister spp. 2 1.12 - anaerobic rod
Peptoniphilus asaccharolyticus 2 1.12 + anaerobic cocci
Enterobacter cloacae 1 0.56 - Facultative anaerobe rod
Klebsiella oxytoca* 1 0.56 - Facultative anaerobe rod
Escherichia coli 1 0.56 - facultative rod
Pseudoalteromonas spp. 1 0.56 - facultative rod
Porphyromonas levii 1 0.56 - anaerobic rod
Delftia acidovorans 1 0.56 - aerobic rod
Dialister invisus 1 0.56 - anaerobic rod
Staphylococcus epidermidis* 1 0.56 + facultative cocci
Staphylococcus spp.* 1 0.56 + facultative cocci
Granulicatella spp. 1 0.56 + anaerobic cocci
  1. This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the D sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the D pool. Bacteria that were cultured and were not found using FRACSS include Pseudomonas, Serratia, and Streptococcus. The % represents the percentage of the total sequences analyzed within the sample.