Table 3 Bacterial strains and plasmids used in this study
From: AI-2-dependent gene regulation in Staphylococcus epidermidis
Strains/plasmids | Relevant genotype and property | Source/reference |
|---|---|---|
S. epidermidis | Â | Â |
1457 | Wild-type strain | [38] |
1457 ΔluxS | luxS mutant (luxS- ermr) | [24] |
E. coli | Â | Â |
XL1 blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZΔM15 Tn10 (Tetr)] | Qiagen |
SG13009 [pREP4] | K12 derivative, Nals Strs Rifs Thi- Lac- Ara- Gal+ Mtl- F- RecA+ Uvr+ Lon+ [pREP4 KanR] | Qiagen |
BL21 | F-,omp T, hsd S(rB-, mB-), gal | Amersham |
V. harveyi | Â | Â |
BB170 | luxN::Tn5(sensor-1- sensor-2+), AI-2 reporter strain | ATCC BAA-1117 |
Plasmids | Â | Â |
pQE-9 | lac Iq, 3.4 kb, Apr, T5, C-terminal 6 × His-tag | Qiagen |
pQE-luxS | pQE-9 containing the luxS gene of S. epidermidis 1457 | this study |
pGEX-4T-1 | lac Iq, 4.9 bp, Apr, GST gene fusion vector | Amersham |
pGEX-pfs | pGEX-4T-1 containing the pfs gene of S. epidermidis 1457 | this study |