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Figure 1 | BMC Microbiology

Figure 1

From: Study of the role of Mce3R on the transcription of mce genes of Mycobacterium tuberculosis

Figure 1

Disruption of the mce3R gene of M. tuberculosis H37Rv. (A) Southern blot analysis of chromosomal DNA from sucR counterselected tuberculosis clone (lane 3) and parental strain (lane 2). Genomic DNA was digested with EcoRI and hybridized to the mce3R probe. Arrows indicate position of hybridizing fragments. MWM 1 kb Promega is shown on the left (lane 1). (B) Restriction map of mutant and wild type strains. The insertion of hygromycin-resistant cassettes is indicated (hyg). Arrows represent the length of expected bands after digestion with EcoRI (E). Value on each arrow indicates the molecular weight of expected bands expressed in base pairs (bp).

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