Development of a novel system for isolating genes involved in predator-prey interactions using host independent derivatives of Bdellovibrio bacteriovorus 109J

Table 1 Genetic location of transposon mutations

Allele*	ORF designation^a Putative function	Location^b	% Identity^c
BPM-5	Bd0530 Flagellar basal-body rod protein- flgE	+213	99 (147)
BPM-6	Bd0115 putative pilus assembly gene cluster	+369	93 (529)
BPM -7	Bd3852 Tfp pilus assembly protein - pilT gene	+61	97 (651)
BPM -8	Bd2503 Methyl-accepting chemotaxis protein	+464	90 (215)
BPM -13	Bd0112 pilus assembly protein secretin-pilQ	+1466	95 (408)
BPM -14	Bd3395 Flagellar hook protein-flgE	+978	99 (741)
BPM -15	Bd0536 Flagellar protein-flgJ	+103	96 (386)
BPM -20	Bd0864 Tfp pilus assembly protein pilN	+523	93 (533)
BPM -28	Bd1481 Adventurous gliding motility protein R aglR	+485	96 (618)
BPM -37	Bd1483 Putative protein in gliding motility cluster	+1022	94 (420)
HI-Ra	Bd3650 Putative Histidine kinase	+1254	94 (866)
HI-Rb	Bd1112 Putative antimicrobial peptide transport	+1259	99 (680)

^a. Designation and putative function from Bdellovibrio bacteriovorus strain HD100 annotation.
^b. Location of the transposon insertion site with respect to the first base pair of the ORF.
^c. % nucleotide identity of transposon flanking sequence to corresponding strain HD100 DNA (number of bp compared).
* BPM number represent lab collection serial number.

ISSN: 1471-2180