Figure 5

Detection of chlamydial Hsp60. HAEC were infected with 10 IFU/cell (A, B) or with 20 IFU/cell and additionally treated with chloramphenicol (C). Cells were stained with anti C. pneumoniae neat serum (red), anti C. pneumoniae Hsp60 (green), DAPI (blue) and colocalization of cHsp60 and MOMP was calculated (white). A: A C. pneumoniae inclusion (asterisk) strongly colocalizes with the cHsp60 signal. In contrast, all C. pneumoniae-spots are negative for the cHsp60 signal. B: The nucleus of spot-like infected HAEC is condensed and many chlamydial spots colocalize with cHsp60. C: Chloramphenicol treated infected HAEC display a regular nuclear morphology and many C. pneumoniae-spots which predominantly do not colocalize with the cHsp60 signal. Scale bar; 10 μm.