Figure 3

CA-SIIF inhibits macrophage IL-12 production both (A) in vitro and (B) in vivo in a murine model. (A) murine peritoneal Macrophages (MØ) were elicited by TG treatment and collected from the peritoneum of C57Bl/6 mice 5 days after elicitation. 2 × 10⁶/ml macrophages were cultured in the presence or absence of LPS (50 ng) and IFN-γ (0.5 ng) stimulation for 16 h with or without 1 mg CA-SIIF. Next, IL-12 levels were measured in the supernatant by ELISA using antibodies specific for murine IL-12 p70. (B) LPS (100 ng) was administered intraperitoneally immediately followed with or without 1 mg CA-SIIF in mice 1 h prior to intravenous injection of LPS (1 μg) to stimulate IL-12 production. Serum IL-12 levels were then measured. Macrophages alone served as controls for base line IL-12 production.