Effects of mixed infection on total titers. Mean titers at 120 hours are shown for each type of infection. Bars indicate standard error of the means. White bars represent single-strain infections where each assay received either DENV2 or DENV4 at a total MOI of 10 (4, 5, 11 and 12, Table 1). Thus, the bar is a mean for single-strain infections for all DENV2 and DENV4 replicates averaged together. Grey bars represent mixed-strain infections where each assay received both DENV2 and DENV4 at a MOI of 5 for each. The superinfection category includes both orders of superinfection (2 then 4, 4 then 2; treatments 13 and 14, Table 1), and only the 6-hour superinfection data are included. Coinfections included treatments 3 and 10 from Table 1. Titers of the entire virus population were significantly lower in superinfection treatments than in single-strain infections (indicated by *).